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Mitochondrial matrix RTN4IP1/OPA10 is an oxidoreductase for coenzyme Q synthesis
DC Field | Value | Language |
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dc.contributor.author | Park, Isaac | - |
dc.contributor.author | Kim, Kwang-eun | - |
dc.contributor.author | Kim, Jeesoo | - |
dc.contributor.author | Kim, Ae-Kyeong | - |
dc.contributor.author | Bae, Subin | - |
dc.contributor.author | Jung, Minkyo | - |
dc.contributor.author | Choi, Jinhyuk | - |
dc.contributor.author | Mishra, Pratyush Kumar | - |
dc.contributor.author | Kim, Taek-Min | - |
dc.contributor.author | Kwak, Chulhwan | - |
dc.contributor.author | Kang, Myeong-Gyun | - |
dc.contributor.author | Yoo, Chang-Mo | - |
dc.contributor.author | Mun, Ji Young | - |
dc.contributor.author | Liu, Kwang-Hyeon | - |
dc.contributor.author | Lee, Kyu-Sun | - |
dc.contributor.author | Kim, Jong-Seo | - |
dc.contributor.author | Suh, Jae Myoung | - |
dc.contributor.author | Rhee, Hyun-Woo | - |
dc.date.accessioned | 2024-05-14T06:47:42Z | - |
dc.date.available | 2024-05-14T06:47:42Z | - |
dc.date.created | 2023-11-13 | - |
dc.date.created | 2023-11-13 | - |
dc.date.issued | 2024-02 | - |
dc.identifier.citation | Nature Chemical Biology, Vol.20 No.2, pp.221-233 | - |
dc.identifier.issn | 1552-4450 | - |
dc.identifier.uri | https://hdl.handle.net/10371/201873 | - |
dc.description.abstract | Targeting proximity-labeling enzymes to specific cellular locations is a viable strategy for profiling subcellular proteomes. Here, we generated transgenic mice (MAX-Tg) expressing a mitochondrial matrix-targeted ascorbate peroxidase. Comparative analysis of matrix proteomes from the muscle tissues showed differential enrichment of mitochondrial proteins. We found that reticulon 4-interacting protein 1 (RTN4IP1), also known as optic atrophy-10, is enriched in the mitochondrial matrix of muscle tissues and is an NADPH oxidoreductase. Interactome analysis and in vitro enzymatic assays revealed an essential role for RTN4IP1 in coenzyme Q (CoQ) biosynthesis by regulating the O-methylation activity of COQ3. Rtn4ip1-knockout myoblasts had markedly decreased CoQ9 levels and impaired cellular respiration. Furthermore, muscle-specific knockdown of dRtn4ip1 in flies resulted in impaired muscle function, which was reversed by dietary supplementation with soluble CoQ. Collectively, these results demonstrate that RTN4IP1 is a mitochondrial NAD(P)H oxidoreductase essential for supporting mitochondrial respiration activity in the muscle tissue. The development of a transgenic mouse line that expresses mitochondrial matrix-targeted APEX2 combined with proteome analysis identified RTN4IP1, which serves as an NAD(P)H oxidoreductase required for respiration and CoQ biosynthesis. | - |
dc.language | 영어 | - |
dc.publisher | Nature Publishing Group | - |
dc.title | Mitochondrial matrix RTN4IP1/OPA10 is an oxidoreductase for coenzyme Q synthesis | - |
dc.type | Article | - |
dc.identifier.doi | 10.1038/s41589-023-01452-w | - |
dc.citation.journaltitle | Nature Chemical Biology | - |
dc.identifier.wosid | 001090044900004 | - |
dc.identifier.scopusid | 2-s2.0-85174964622 | - |
dc.citation.endpage | 233 | - |
dc.citation.number | 2 | - |
dc.citation.startpage | 221 | - |
dc.citation.volume | 20 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Kim, Jong-Seo | - |
dc.contributor.affiliatedAuthor | Rhee, Hyun-Woo | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | SACCHAROMYCES-CEREVISIAE | - |
dc.subject.keywordPlus | MUSCLE | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | METHYLTRANSFERASE | - |
dc.subject.keywordPlus | METABOLISM | - |
dc.subject.keywordPlus | UBIQUINONE | - |
dc.subject.keywordPlus | MUTATIONS | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
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