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Resin-Assisted Enrichment of N-Terminal Peptides for Characterizing Proteolytic Processing
DC Field | Value | Language |
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dc.contributor.author | Kim, Jong-Seo | - |
dc.contributor.author | Dai, Ziyu | - |
dc.contributor.author | Aryal, Uma K. | - |
dc.contributor.author | Moore, Ronald J. | - |
dc.contributor.author | Camp, David G., II | - |
dc.contributor.author | Baker, Scott E. | - |
dc.contributor.author | Smith, Richard D. | - |
dc.contributor.author | Qian, Wei-Jun | - |
dc.date.accessioned | 2024-05-14T06:48:58Z | - |
dc.date.available | 2024-05-14T06:48:58Z | - |
dc.date.created | 2023-06-26 | - |
dc.date.issued | 2013-07 | - |
dc.identifier.citation | Analytical Chemistry, Vol.85 No.14, pp.6826-6832 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.uri | https://hdl.handle.net/10371/201895 | - |
dc.description.abstract | A resin-assisted enrichment method has been developed for specific isolation of protein N-terminal peptides to facilitate LC-MS/MS characterization of proteolytic processing, a major form of posttranslational modifications. In this method, protein thiols are blocked by reduction and alkylation, and protein lysine residues are converted to homoarginines. Protein N-termini are selectively converted to reactive thiol groups, and the thiol-containing N-terminal peptides are then captured by a thiol-affinity resin with high specificity (>97%). The efficiencies of these sequential reactions were demonstrated to be nearly quantitative. The resin-assisted N-terminal peptide enrichment approach was initially applied to a cell lysate of the filamentous fungus Aspergillus niger. Subsequent C-MS/MS analyses resulted in the identification of 1672 unique protein N-termini or proteolytic cleavage sites from 690 unique proteins. | - |
dc.language | 영어 | - |
dc.publisher | American Chemical Society | - |
dc.title | Resin-Assisted Enrichment of N-Terminal Peptides for Characterizing Proteolytic Processing | - |
dc.type | Article | - |
dc.identifier.doi | 10.1021/ac401000q | - |
dc.citation.journaltitle | Analytical Chemistry | - |
dc.identifier.wosid | 000322059600040 | - |
dc.identifier.scopusid | 2-s2.0-84880519251 | - |
dc.citation.endpage | 6832 | - |
dc.citation.number | 14 | - |
dc.citation.startpage | 6826 | - |
dc.citation.volume | 85 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Kim, Jong-Seo | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | TANDEM MASS-SPECTRA | - |
dc.subject.keywordPlus | SYSTEMATIC IDENTIFICATION | - |
dc.subject.keywordPlus | POSITIONAL PROTEOMICS | - |
dc.subject.keywordPlus | CLEAVAGE SITES | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | DERIVATIZATION | - |
dc.subject.keywordPlus | THROUGHPUT | - |
dc.subject.keywordPlus | STRATEGY | - |
dc.subject.keywordPlus | SIGNALP | - |
dc.subject.keywordPlus | SCALE | - |
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