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Antibody-free, targeted mass-spectrometric approach for quantification of proteins at low picogram per milliliter levels in human plasma/serum
DC Field | Value | Language |
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dc.contributor.author | Shi, Tujin | - |
dc.contributor.author | Fillmore, Thomas L. | - |
dc.contributor.author | Sun, Xuefei | - |
dc.contributor.author | Zhao, Rui | - |
dc.contributor.author | Schepmoes, Athena A. | - |
dc.contributor.author | Hossain, Mahmud | - |
dc.contributor.author | Xie, Fang | - |
dc.contributor.author | Wu, Si | - |
dc.contributor.author | Kim, Jong-Seo | - |
dc.contributor.author | Jones, Nathan | - |
dc.contributor.author | Moore, Ronald J. | - |
dc.contributor.author | Pasa-Tolic, Ljiljana | - |
dc.contributor.author | Kagan, Jacob | - |
dc.contributor.author | Rodland, Karin D. | - |
dc.contributor.author | Liu, Tao | - |
dc.contributor.author | Tang, Keqi | - |
dc.contributor.author | Camp, David G., II | - |
dc.contributor.author | Smith, Richard D. | - |
dc.contributor.author | Qian, Wei-Jun | - |
dc.date.accessioned | 2024-05-14T06:49:08Z | - |
dc.date.available | 2024-05-14T06:49:08Z | - |
dc.date.created | 2023-06-26 | - |
dc.date.issued | 2012-09 | - |
dc.identifier.citation | Proceedings of the National Academy of Sciences of the United States of America, Vol.109 No.38, pp.15395-15400 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | https://hdl.handle.net/10371/201898 | - |
dc.description.abstract | Sensitive detection of low-abundance proteins in complex biological samples has typically been achieved by immunoassays that use antibodies specific to target proteins; however, de novo development of antibodies is associated with high costs, long development lead times, and high failure rates. To address these challenges, we developed an antibody-free strategy that involves PRISM (high-pressure, high-resolution separations coupled with intelligent selection and multiplexing) for sensitive selected reaction monitoring (SRM)-based targeted protein quantification. The strategy capitalizes on high-resolution reversed-phase liquid chromatographic separations for analyte enrichment, intelligent selection of target fractions via on-line SRM monitoring of internal standards, and fraction multiplexing before nano-liquid chromatography-SRM quantification. Application of this strategy to human plasma/serum demonstrated accurate and reproducible quantification of proteins at concentrations in the 50-100 pg/mL range, which represents a major advance in the sensitivity of targeted protein quantification without the need for specific-affinity reagents. Application to a set of clinical serum samples illustrated an excellent correlation between the results obtained from the PRISM-SRM assay and those from clinical immunoassay for the prostate-specific antigen level. | - |
dc.language | 영어 | - |
dc.publisher | National Academy of Sciences | - |
dc.title | Antibody-free, targeted mass-spectrometric approach for quantification of proteins at low picogram per milliliter levels in human plasma/serum | - |
dc.type | Article | - |
dc.identifier.doi | 10.1073/pnas.1204366109 | - |
dc.citation.journaltitle | Proceedings of the National Academy of Sciences of the United States of America | - |
dc.identifier.wosid | 000309211000067 | - |
dc.identifier.scopusid | 2-s2.0-84866538934 | - |
dc.citation.endpage | 15400 | - |
dc.citation.number | 38 | - |
dc.citation.startpage | 15395 | - |
dc.citation.volume | 109 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Kim, Jong-Seo | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | PROSTATE-SPECIFIC ANTIGEN | - |
dc.subject.keywordPlus | PEPTIDE IMMUNOAFFINITY ENRICHMENT | - |
dc.subject.keywordPlus | QUANTITATIVE PROTEOMICS | - |
dc.subject.keywordPlus | MULTIPLEXED ASSAYS | - |
dc.subject.keywordPlus | FREE PSA | - |
dc.subject.keywordPlus | BIOMARKERS | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | CHROMATOGRAPHY | - |
dc.subject.keywordPlus | VERIFICATION | - |
dc.subject.keywordPlus | SENSITIVITY | - |
dc.subject.keywordAuthor | biomarker verification | - |
dc.subject.keywordAuthor | systems biology | - |
dc.subject.keywordAuthor | targeted proteomics | - |
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