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De novo analysis of protein N-terminal sequence utilizing MALDI signal enhancing derivatization with Br signature
DC Field | Value | Language |
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dc.contributor.author | Kim, Jong-Seo | - |
dc.contributor.author | Song, Jin-Su | - |
dc.contributor.author | Kim, Yongju | - |
dc.contributor.author | Park, Seung Bum | - |
dc.contributor.author | Kim, Hie-Joon | - |
dc.date.accessioned | 2024-05-14T06:49:15Z | - |
dc.date.available | 2024-05-14T06:49:15Z | - |
dc.date.created | 2020-08-20 | - |
dc.date.issued | 2012-02 | - |
dc.identifier.citation | Analytical and Bioanalytical Chemistry, Vol.402 No.5, pp.1911-1919 | - |
dc.identifier.issn | 1618-2642 | - |
dc.identifier.uri | https://hdl.handle.net/10371/201900 | - |
dc.description.abstract | De novo analysis of protein N-terminal sequence is important for identification of N-terminal proteolytic processing such as N-terminal methionine or signal peptide removal, or for the genome annotation of uncharacterized proteins. We introduce a de novo sequencing method of protein N terminus utilizing matrix-assisted laser desorption/ionization (MALDI) signal enhancing picolinamidination with bromine isotopic tag incorporated to the N terminus. The doublet signature of bromine in the tandem mass (MS/MS) spectrum distinguished N-terminal ion series from C-terminal ion series, facilitating de novo N-terminal sequencing of protein. The dual advantage of MALDI signal enhancement by the basic picolinamidine and b-ion selection aided by Br signature is demonstrated using a variety of peptides. The N-terminal sequences of myoglobin and hemoglobin as model proteins were determined by incorporating the Br tag to the N terminus of the proteins and obtaining a series of b-ions with Br signature by MS/MS analysis after chymotryptic digestion of the tagged proteins. The N-terminal peptide was selected for MS/MS analysis from the chymotryptic digest based on the Br signature in the mass spectrum. Identification of phosphorylation site as well as N-terminal sequencing of a phosphopeptide was straightforward. | - |
dc.language | 영어 | - |
dc.publisher | Springer Verlag | - |
dc.title | De novo analysis of protein N-terminal sequence utilizing MALDI signal enhancing derivatization with Br signature | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/s00216-011-5642-7 | - |
dc.citation.journaltitle | Analytical and Bioanalytical Chemistry | - |
dc.identifier.wosid | 000299840700017 | - |
dc.identifier.scopusid | 2-s2.0-84858706385 | - |
dc.citation.endpage | 1919 | - |
dc.citation.number | 5 | - |
dc.citation.startpage | 1911 | - |
dc.citation.volume | 402 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Kim, Jong-Seo | - |
dc.contributor.affiliatedAuthor | Park, Seung Bum | - |
dc.contributor.affiliatedAuthor | Kim, Hie-Joon | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | IONIZATION MASS-SPECTROMETRY | - |
dc.subject.keywordPlus | PICOMOLE-SCALE METHOD | - |
dc.subject.keywordPlus | CHARGE DERIVATIZATION | - |
dc.subject.keywordPlus | 4-SULFOPHENYL ISOTHIOCYANATE | - |
dc.subject.keywordPlus | SULFONATED PEPTIDES | - |
dc.subject.keywordPlus | CARBOXYPEPTIDASE-Y | - |
dc.subject.keywordPlus | BROMINE SIGNATURE | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | FRAGMENTATION | - |
dc.subject.keywordAuthor | De novo sequencing | - |
dc.subject.keywordAuthor | N-terminal | - |
dc.subject.keywordAuthor | Amidination | - |
dc.subject.keywordAuthor | Bromine signature | - |
dc.subject.keywordAuthor | MALDI signal enhancement | - |
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