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Picolinamidination of Phosphopeptides for MALDI-TOF-TOF Mass Spectrometric Sequencing with Enhanced Sensitivity
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Cited 13 time in Scopus
- Authors
- Issue Date
- 2009-09
- Publisher
- Elsevier BV
- Citation
- Journal of the American Society for Mass Spectrometry, Vol.20 No.9, pp.1751-1758
- Abstract
- Two orders of magnitude matrix-assisted laser desorption/ionization (MALDI) signal enhancement of phosphopeptides has been achieved by picolinamidination of N-terminal amine group and epsilon-amine group of lysine residues. Due to die presence of picolinamidination tag at the N-terminal amine of peptides, MS/MS spectra with a strong b-ion series was obtained, which greatly facilitated sequencing and identification of the phosphorylation site. Phosphorylation site of a phosphopeptide could be identified from MALDI TOF/TOF spectrum obtained from a tryptic or a chymotryptic phosphopeptide, which was not even detected in the positive ion mode, without signal enhancement by picolinamidination, due to the negative charge of the phosphate group in the presence of other peptides. (J Am Soc Mass Spectrom 2009,20,1751-1758) (C) 2009 American Society for Mass Spectrometry
- ISSN
- 1044-0305
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