Publications

Detailed Information

Matrix-assisted laser desorption/ionization signal enhancement of peptides by picolinamidination of amino groups

Cited 13 time in Web of Science Cited 14 time in Scopus
Authors

Kim, Jong-Seo; Kim, Ji-Hyun; Kim, Hie-Joon

Issue Date
2008-01
Publisher
John Wiley & Sons Inc.
Citation
Rapid Communications in Mass Spectrometry, Vol.22 No.4, pp.495-502
Abstract
Picolinamidination of amino groups in peptides was carried out using ethyl picolinimidate tetrafluoroborate synthesized from picolinamide and triethyloxonium tetrafluoroborate. The N-terminal amino group as well as the e-amino group of lysine were derivatized. The matrix-assisted laser desorption/ionization (MALDI) signal of a peptide was enhanced 20-35-fold upon picolinamidination depending on the number of amino groups derivatized. The signal enhancement effect is much higher than that of acetamidination or guanidination previously reported. Improved protein identification by mass mapping of the derivatized peptides was demonstrated. Copyright (C) 2008 John Wiley & Sons, Ltd.
ISSN
0951-4198
URI
https://hdl.handle.net/10371/201909
DOI
https://doi.org/10.1002/rcm.3392
Files in This Item:
There are no files associated with this item.
Appears in Collections:

Related Researcher

  • College of Natural Sciences
  • School of Biological Sciences
Research Area Molecular Interactomics, Proteomics, Systems Biology, 단백체학, 분자상호작용체학, 시스템생물학

Altmetrics

Item View & Download Count

  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Share