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Suppression of matrix clusters and enhancement of peptide signals in MALDI-TOF mass spectrometry using nitrilotriacetic acid
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, JS | - |
dc.contributor.author | Kim, JY | - |
dc.contributor.author | Kim, HJ | - |
dc.date.accessioned | 2024-05-14T06:49:51Z | - |
dc.date.available | 2024-05-14T06:49:51Z | - |
dc.date.created | 2024-04-15 | - |
dc.date.issued | 2005-11 | - |
dc.identifier.citation | Analytical Chemistry, Vol.77 No.22, pp.7483-7488 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.uri | https://hdl.handle.net/10371/201910 | - |
dc.description.abstract | Matrix clusters and their alkali metal ion adducts suppress peptide signals in the 500-1400 Da range and compromise MALDI-TOF mass spectrometric peptide mass fingerprinting and protein identification. Addition of 7 mM nitrilotriacetic acid to the matrix solution significantly reduced matrix clusters and increased signal-to-noise ratio of peptide signals similar to 5 to 20-fold. As a result, reliability in the identification of femtomole amounts of proteins based on peptide mass fingerprinting and database search was significantly enhanced, leading to a higher score and sequence coverage. | - |
dc.language | 영어 | - |
dc.publisher | American Chemical Society | - |
dc.title | Suppression of matrix clusters and enhancement of peptide signals in MALDI-TOF mass spectrometry using nitrilotriacetic acid | - |
dc.type | Article | - |
dc.identifier.doi | 10.1021/ac051152l | - |
dc.citation.journaltitle | Analytical Chemistry | - |
dc.identifier.wosid | 000233404200050 | - |
dc.identifier.scopusid | 2-s2.0-27944448483 | - |
dc.citation.endpage | 7488 | - |
dc.citation.number | 22 | - |
dc.citation.startpage | 7483 | - |
dc.citation.volume | 77 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Kim, JS | - |
dc.contributor.affiliatedAuthor | Kim, HJ | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | ASSISTED-LASER-DESORPTION/IONIZATION | - |
dc.subject.keywordPlus | ALPHA-CYANO-4-HYDROXYCINNAMIC ACID | - |
dc.subject.keywordPlus | QUANTITATIVE MODEL | - |
dc.subject.keywordPlus | ION FORMATION | - |
dc.subject.keywordPlus | MS ANALYSIS | - |
dc.subject.keywordPlus | IONIZATION | - |
dc.subject.keywordPlus | PROTEINS | - |
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