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Novel turn-on fluorescent biosensors for selective detection of cellular Fe3+ in lysosomes: Thiophene as a selectivity-tuning handle for Fe3+ sensors

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dc.contributor.authorLim, Bumhee-
dc.contributor.authorBaek, Byungyeob-
dc.contributor.authorJang, Kyungkuk-
dc.contributor.authorLee, Na Keum-
dc.contributor.authorLee, Ji Hye-
dc.contributor.authorLee, Yeongcheol-
dc.contributor.authorKim, Jinwoo-
dc.contributor.authorKang, San Won-
dc.contributor.authorPark, Jaehyun-
dc.contributor.authorKim, Suzi-
dc.contributor.authorKang, Nae-Won-
dc.contributor.authorHong, Suckchang-
dc.contributor.authorKim, Dae-Duk-
dc.contributor.authorKim, Ikyon-
dc.contributor.authorHwang, Hyonseok-
dc.contributor.authorLee, Jeeyeon-
dc.date.accessioned2024-05-14T07:17:30Z-
dc.date.available2024-05-14T07:17:30Z-
dc.date.created2019-10-23-
dc.date.issued2019-10-
dc.identifier.citationDyes and Pigments, Vol.169, pp.51-59-
dc.identifier.issn0143-7208-
dc.identifier.urihttps://hdl.handle.net/10371/202011-
dc.description.abstractIron-selective turn-on sensors are indispensable tools for understanding iron-related cell death processes and human diseases. In this study, we report a novel class of fluorescent sensors derived from an indolizino[3,2-c]quinoline scaffold that exhibit high selectivity for Fe3+ over other biologically abundant cations in cells, including Fe2+, Al3+, Zn2+, and Mn2+. IQ18 works as a ratiometric sensor with a K-d value of 7.1 x 10(-7)M and a detection limit of 5.2 nM in ethanol, whereas IQ44 displays fluorescence enhancement upon binding with Fe3+ in both ethanol and water. In aqueous solution, IQ44 exists as 150-nm nanoparticles. The suppressed fluorescent emission of IQ44 nanoparticles in water is switched on in response to Fe3+, working as a turn-on nanoparticle sensor. Structure-property relationship analysis with IQ derivatives revealed that the thiophene ring confers selectivity for Fe3+. By installing thiophene in IQ44 as a selectivity-tuning handle, fluorescence in the presence of Fe3+ resulting from restriction of intramolecular rotation (RIR) and increased torsion angle induced by iron demonstrated that IQ44 is specifically localized in lysosotiies, where it recognizes cellular Fe3+ in live cells, as determined using confocal microscopy. In addition, the increased fluorescent puncta of IQ44 in the presence of Fe3+ colocalized well with the RFP-tagged LC3 proteins (pmRFP-LC3), enabling the detection of the autophagy process.-
dc.language영어-
dc.publisherElsevier BV-
dc.titleNovel turn-on fluorescent biosensors for selective detection of cellular Fe3+ in lysosomes: Thiophene as a selectivity-tuning handle for Fe3+ sensors-
dc.typeArticle-
dc.identifier.doi10.1016/j.dyepig.2019.05.008-
dc.citation.journaltitleDyes and Pigments-
dc.identifier.wosid000473380500008-
dc.identifier.scopusid2-s2.0-85065512672-
dc.citation.endpage59-
dc.citation.startpage51-
dc.citation.volume169-
dc.description.isOpenAccessN-
dc.contributor.affiliatedAuthorHong, Suckchang-
dc.contributor.affiliatedAuthorKim, Dae-Duk-
dc.contributor.affiliatedAuthorLee, Jeeyeon-
dc.type.docTypeArticle-
dc.description.journalClass1-
dc.subject.keywordPlusAGGREGATION-INDUCED EMISSION-
dc.subject.keywordPlusORGANIC NANOPARTICLES-
dc.subject.keywordPlusMULTICOMPONENT REACTION-
dc.subject.keywordPlusINTRALYSOSOMAL IRON-
dc.subject.keywordPlusFACILE FABRICATION-
dc.subject.keywordPlusOXIDATIVE STRESS-
dc.subject.keywordPlusFAR-RED-
dc.subject.keywordPlusIRON(III)-
dc.subject.keywordPlusPROBES-
dc.subject.keywordPlusAIE-
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  • College of Pharmacy
  • Department of Manufacturing Pharmacy
Research Area Development of methodologies using metal catalyst, Total synthesis of natural products

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