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Novel turn-on fluorescent biosensors for selective detection of cellular Fe3+ in lysosomes: Thiophene as a selectivity-tuning handle for Fe3+ sensors
DC Field | Value | Language |
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dc.contributor.author | Lim, Bumhee | - |
dc.contributor.author | Baek, Byungyeob | - |
dc.contributor.author | Jang, Kyungkuk | - |
dc.contributor.author | Lee, Na Keum | - |
dc.contributor.author | Lee, Ji Hye | - |
dc.contributor.author | Lee, Yeongcheol | - |
dc.contributor.author | Kim, Jinwoo | - |
dc.contributor.author | Kang, San Won | - |
dc.contributor.author | Park, Jaehyun | - |
dc.contributor.author | Kim, Suzi | - |
dc.contributor.author | Kang, Nae-Won | - |
dc.contributor.author | Hong, Suckchang | - |
dc.contributor.author | Kim, Dae-Duk | - |
dc.contributor.author | Kim, Ikyon | - |
dc.contributor.author | Hwang, Hyonseok | - |
dc.contributor.author | Lee, Jeeyeon | - |
dc.date.accessioned | 2024-05-14T07:17:30Z | - |
dc.date.available | 2024-05-14T07:17:30Z | - |
dc.date.created | 2019-10-23 | - |
dc.date.issued | 2019-10 | - |
dc.identifier.citation | Dyes and Pigments, Vol.169, pp.51-59 | - |
dc.identifier.issn | 0143-7208 | - |
dc.identifier.uri | https://hdl.handle.net/10371/202011 | - |
dc.description.abstract | Iron-selective turn-on sensors are indispensable tools for understanding iron-related cell death processes and human diseases. In this study, we report a novel class of fluorescent sensors derived from an indolizino[3,2-c]quinoline scaffold that exhibit high selectivity for Fe3+ over other biologically abundant cations in cells, including Fe2+, Al3+, Zn2+, and Mn2+. IQ18 works as a ratiometric sensor with a K-d value of 7.1 x 10(-7)M and a detection limit of 5.2 nM in ethanol, whereas IQ44 displays fluorescence enhancement upon binding with Fe3+ in both ethanol and water. In aqueous solution, IQ44 exists as 150-nm nanoparticles. The suppressed fluorescent emission of IQ44 nanoparticles in water is switched on in response to Fe3+, working as a turn-on nanoparticle sensor. Structure-property relationship analysis with IQ derivatives revealed that the thiophene ring confers selectivity for Fe3+. By installing thiophene in IQ44 as a selectivity-tuning handle, fluorescence in the presence of Fe3+ resulting from restriction of intramolecular rotation (RIR) and increased torsion angle induced by iron demonstrated that IQ44 is specifically localized in lysosotiies, where it recognizes cellular Fe3+ in live cells, as determined using confocal microscopy. In addition, the increased fluorescent puncta of IQ44 in the presence of Fe3+ colocalized well with the RFP-tagged LC3 proteins (pmRFP-LC3), enabling the detection of the autophagy process. | - |
dc.language | 영어 | - |
dc.publisher | Elsevier BV | - |
dc.title | Novel turn-on fluorescent biosensors for selective detection of cellular Fe3+ in lysosomes: Thiophene as a selectivity-tuning handle for Fe3+ sensors | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.dyepig.2019.05.008 | - |
dc.citation.journaltitle | Dyes and Pigments | - |
dc.identifier.wosid | 000473380500008 | - |
dc.identifier.scopusid | 2-s2.0-85065512672 | - |
dc.citation.endpage | 59 | - |
dc.citation.startpage | 51 | - |
dc.citation.volume | 169 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Hong, Suckchang | - |
dc.contributor.affiliatedAuthor | Kim, Dae-Duk | - |
dc.contributor.affiliatedAuthor | Lee, Jeeyeon | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | AGGREGATION-INDUCED EMISSION | - |
dc.subject.keywordPlus | ORGANIC NANOPARTICLES | - |
dc.subject.keywordPlus | MULTICOMPONENT REACTION | - |
dc.subject.keywordPlus | INTRALYSOSOMAL IRON | - |
dc.subject.keywordPlus | FACILE FABRICATION | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | FAR-RED | - |
dc.subject.keywordPlus | IRON(III) | - |
dc.subject.keywordPlus | PROBES | - |
dc.subject.keywordPlus | AIE | - |
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