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Upgraded User-Friendly Image-Activated Microfluidic Cell Sorter Using an Optimized and Fast Deep Learning Algorithm

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Authors

Lee, Keondo; Kim, Seong-Eun; Nam, Seokho; Doh, Junsang; Chung, Wan Kyun

Issue Date
2022-12
Publisher
Multidisciplinary Digital Publishing Institute (MDPI)
Citation
Micromachines, Vol.13 No.12, p. mi13122105
Abstract
Image-based cell sorting is essential in biological and biomedical research. The sorted cells can be used for downstream analysis to expand our knowledge of cell-to-cell differences. We previously demonstrated a user-friendly image-activated microfluidic cell sorting technique using an optimized and fast deep learning algorithm. Real-time isolation of cells was carried out using this technique with an inverted microscope. In this study, we devised a recently upgraded sorting system. The cell sorting techniques shown on the microscope were implemented as a real system. Several new features were added to make it easier for the users to conduct the real-time sorting of cells or particles. The newly added features are as follows: (1) a high-resolution linear piezo-stage is used to obtain in-focus images of the fast-flowing cells; (2) an LED strobe light was incorporated to minimize the motion blur of fast-flowing cells; and (3) a vertical syringe pump setup was used to prevent the cell sedimentation. The sorting performance of the upgraded system was demonstrated through the real-time sorting of fluorescent polystyrene beads. The sorter achieved a 99.4% sorting purity for 15 mu m and 10 mu m beads with an average throughput of 22.1 events per second (eps).
ISSN
2072-666X
URI
https://hdl.handle.net/10371/202432
DOI
https://doi.org/10.3390/mi13122105
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