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Establishment of patient-derived organotypic tumor spheroid models for tumor microenvironment modeling
DC Field | Value | Language |
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dc.contributor.author | Hong, Hye Kyung | - |
dc.contributor.author | Yun, Nak Hyeon | - |
dc.contributor.author | Jeong, Ye-Lin | - |
dc.contributor.author | Park, Jeehun | - |
dc.contributor.author | Doh, Junsang | - |
dc.contributor.author | Lee, Woo Yong | - |
dc.contributor.author | Cho, Yong Beom | - |
dc.date.accessioned | 2024-05-16T01:21:31Z | - |
dc.date.available | 2024-05-16T01:21:31Z | - |
dc.date.created | 2021-07-28 | - |
dc.date.created | 2021-07-28 | - |
dc.date.created | 2021-07-28 | - |
dc.date.issued | 2021-08 | - |
dc.identifier.citation | Cancer Medicine, Vol.10 No.16, pp.5589-5598 | - |
dc.identifier.issn | 2045-7634 | - |
dc.identifier.uri | https://hdl.handle.net/10371/202455 | - |
dc.description.abstract | Patient-derived cancer models that reconstitute the characteristics of the tumor microenvironment may facilitate efforts in precision immune-oncology and the discovery of effective anticancer therapies. Organoids that have recently emerged as robust preclinical models typically contain tumor epithelial cells and lack the native tumor immune microenvironment. A patient-derived organotypic tumor spheroid (PDOTS) is a novel and innovative ex vivo system that retains key features of the native tumor immune microenvironment. Here, we established and characterized a series of colorectal cancer PDOTS models for use as a preclinical platform for testing effective immunotherapy and its combinations with other drugs. Partially dissociated (> 100 mu m in diameter) tumor tissues were embedded in Matrigel-containing organoid media and subsequently formed into organoid structures within 3 to 7 days of culture. The success rate of growing PDOTS from fresh tissues was similar to 86%. Morphological analysis showed that the PDOTSs varied in size and structure. Immunofluorescence and flow cytometry analysis revealed that the PDOTSs retained autologous tumor-infiltrating lymphoid cells and tumor-infiltrating lymphoid cells were continually decreased through serial passages. Notably, PDOTSs from tumors from a high-level microsatellite instability-harboring patient were sensitive to anti-PD-1 or anti-PD-L1 antibodies. Our results demonstrate that the PDOTS model in which the tumor immune microenvironment is preserved may represent an advantageous ex vivo system to develop effective immune therapeutics. | - |
dc.language | 영어 | - |
dc.publisher | John Wiley and Sons Ltd | - |
dc.title | Establishment of patient-derived organotypic tumor spheroid models for tumor microenvironment modeling | - |
dc.type | Article | - |
dc.identifier.doi | 10.1002/cam4.4114 | - |
dc.citation.journaltitle | Cancer Medicine | - |
dc.identifier.wosid | 000670864800001 | - |
dc.identifier.scopusid | 2-s2.0-85109734121 | - |
dc.citation.endpage | 5598 | - |
dc.citation.number | 16 | - |
dc.citation.startpage | 5589 | - |
dc.citation.volume | 10 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Doh, Junsang | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | ORGANOID MODELS | - |
dc.subject.keywordPlus | HUMAN COLON | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | BLOCKADE | - |
dc.subject.keywordAuthor | colorectal cancer | - |
dc.subject.keywordAuthor | immune therapeutics | - |
dc.subject.keywordAuthor | organotypic tumor spheroid | - |
dc.subject.keywordAuthor | patient-derived cancer model | - |
dc.subject.keywordAuthor | tumor microenvironment | - |
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