Publications

Detailed Information

Corneal toxicity of topical tacrolimus ointment in mice with corneal epithelial injury

Cited 3 time in Web of Science Cited 3 time in Scopus
Authors

Yoon, Chang Ho; Park, Jong Woo; Ryu, Jin Suk; Kim, Mee Kum; Oh, Joo Youn

Issue Date
2018-12
Publisher
Mary Ann Liebert Inc.
Citation
Journal of Ocular Pharmacology and Therapeutics, Vol.34 No.10, pp.685-691
Abstract
Purpose: To investigate the effect of tacrolimus ointment on corneal epithelium. Methods: Eight-week-old male BALB/c mice were divided into 3 groups. In group 1, no injury was made. In group 2, the central 2-mm corneal epithelium was scraped off. In group 3, the whole corneal and limbal epithelium was removed after absolute ethanol application. In each group, the corneas were observed and treated daily with 0.03% or 0.1% tacrolimus ointment (Protopic((R))). The viability of cultivated human corneal epithelial cells was also examined after 24-h incubation with various concentrations of tacrolimus. Results: Tacrolimus ointment significantly delayed the epithelial healing in corneas with epithelial injuries (groups 2 and 3) in dose- and frequency-dependent manners, whereas it did not have any effects on uninjured corneas (group 1). The proportion of terminal deoxynucleotidyl transferase-mediated nick end labeling-positive cells and mRNA levels of inflammatory cytokines were higher in tacrolimus-treated corneas, compared with controls. Tacrolimus did not directly reduce the viability of corneal epithelial cells in culture. Conclusions: Topical application of tacrolimus ointment onto the ocular surface induced toxicity in the cornea with epithelial defects through the impairment of epithelial healing and induction of apoptosis.
ISSN
1080-7683
URI
https://hdl.handle.net/10371/202840
DOI
https://doi.org/10.1089/jop.2018.0054
Files in This Item:
There are no files associated with this item.
Appears in Collections:

Related Researcher

  • College of Medicine
  • Department of Medicine
Research Area 각막 및 외안부 질환, 백내장

Altmetrics

Item View & Download Count

  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Share