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Microarray-based analysis of gene expression profiles in peripheral blood of patients with acute primary angle closure
DC Field | Value | Language |
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dc.contributor.author | Jeoung, Jin Wook | - |
dc.contributor.author | Ko, Jung Hwa | - |
dc.contributor.author | Kim, Yu Jeong | - |
dc.contributor.author | Kim, Yong Woo | - |
dc.contributor.author | Park, Ki Ho | - |
dc.contributor.author | Oh, Joo Youn | - |
dc.date.accessioned | 2024-05-16T01:47:21Z | - |
dc.date.available | 2024-05-16T01:47:21Z | - |
dc.date.created | 2018-11-13 | - |
dc.date.created | 2018-11-13 | - |
dc.date.created | 2018-11-13 | - |
dc.date.issued | 2017-11 | - |
dc.identifier.citation | Ophthalmic Genetics, Vol.38 No.6, pp.520-526 | - |
dc.identifier.issn | 1381-6810 | - |
dc.identifier.uri | https://hdl.handle.net/10371/202860 | - |
dc.description.abstract | Background: We investigated the expression of molecules in peripheral blood mononuclear cells (PBMCs) and plasma of patients with acute primary angle closure (APAC). Materials and methods: Peripheral blood was collected from patients with APAC (n = 10) and age-matched controls (n = 5). The gene transcription profile was analyzed in PBMCs using microarrays and validated by real-time reverse transcription polymerase chain reaction (RT-PCR). The levels of secreted proteins were evaluated in plasma by ELISA. Results: 347 gene transcripts were up-regulated by 2-fold or more, and 696 transcripts down-regulated 2-fold or more in PBMCs from patients compared to controls. The most highly up-regulated gene was thrombospondin-1 (TSP-1, 8.66-fold increase), and the most down-regulated gene was prostaglandin-endoperoxide synthase 2 (PTGS2, 9.09-fold decrease). Real-time RT-PCR assay confirmed the increase of TSP-1 and the decrease of PTGS2 in PBMCs of patients. ELISA revealed that the levels of TSP-1 and active transforming growth factor (TGF)-β1 that is activated by TSP-1 were elevated in plasma of patients, while the level of prostaglandin E2 (PGE2) that is converted by PTGS2 was reduced. The plasma level of TSP-1 was positively correlated with that of active TGF-β1. Conclusions: Our data suggest that the molecular network including TSP-1, TGF-β1, and PGE2 might be involved in the pathogenesis of APAC and PACG. © 2017 Taylor & Francis. | - |
dc.language | 영어 | - |
dc.publisher | Swets & Zeitlinger | - |
dc.title | Microarray-based analysis of gene expression profiles in peripheral blood of patients with acute primary angle closure | - |
dc.type | Article | - |
dc.identifier.doi | 10.1080/13816810.2017.1300922 | - |
dc.citation.journaltitle | Ophthalmic Genetics | - |
dc.identifier.wosid | 000418050900003 | - |
dc.identifier.scopusid | 2-s2.0-85015785890 | - |
dc.citation.endpage | 526 | - |
dc.citation.number | 6 | - |
dc.citation.startpage | 520 | - |
dc.citation.volume | 38 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Park, Ki Ho | - |
dc.contributor.affiliatedAuthor | Oh, Joo Youn | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | AQUEOUS-HUMOR | - |
dc.subject.keywordPlus | TRABECULAR MESHWORK | - |
dc.subject.keywordPlus | GLAUCOMATOUS EYES | - |
dc.subject.keywordPlus | THROMBOSPONDIN-1 | - |
dc.subject.keywordPlus | METAANALYSIS | - |
dc.subject.keywordPlus | MECHANISMS | - |
dc.subject.keywordPlus | TGF-BETA-1 | - |
dc.subject.keywordPlus | CYTOKINES | - |
dc.subject.keywordPlus | PRESSURE | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordAuthor | Acute primary angle closure | - |
dc.subject.keywordAuthor | peripheral blood mononuclear cells | - |
dc.subject.keywordAuthor | prostaglandin E2 | - |
dc.subject.keywordAuthor | thrombospondin-1 | - |
dc.subject.keywordAuthor | transforming growth factor-1 | - |
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