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Structural insights into ubiquitin chain cleavage by Legionella ovarian tumor deubiquitinases
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kang, Sangwoo | - |
dc.contributor.author | Kim, Gyuhee | - |
dc.contributor.author | Choi, Minhyeong | - |
dc.contributor.author | Jeong, Minwoo | - |
dc.contributor.author | van Noort, Gerbrand J. van der Heden | - |
dc.contributor.author | Roh, Soung-Hun | - |
dc.contributor.author | Shin, Donghyuk | - |
dc.date.accessioned | 2024-05-16T01:49:46Z | - |
dc.date.available | 2024-05-16T01:49:46Z | - |
dc.date.created | 2023-05-23 | - |
dc.date.created | 2023-05-23 | - |
dc.date.issued | 2023-07 | - |
dc.identifier.citation | Life Science Alliance, Vol.6 No.7 | - |
dc.identifier.issn | 2575-1077 | - |
dc.identifier.uri | https://hdl.handle.net/10371/202895 | - |
dc.description.abstract | Although ubiquitin is found only in eukaryotes, several patho-genic bacteria and viruses possess proteins that hinder the host ubiquitin system. Legionella, a gram-negative intracellular bacterium, possesses an ovarian tumor (OTU) family of deubi-quitinases (Lot DUBs). Herein, we describe the molecular char-acteristics of Lot DUBs. We elucidated the structure of the LotA OTU1 domain and revealed that entire Lot DUBs possess a characteristic extended helical lobe that is not found in other OTU-DUBs. The structural topology of an extended helical lobe is the same throughout the Lot family, and it provides an S19 ubiquitin-binding site. Moreover, the catalytic triads of Lot DUBs resemble those of the A20-type OTU-DUBs. Furthermore, we revealed a unique mechanism by which LotA OTU domains co-operate together to distinguish the length of the chain and preferentially cleave longer K48-linked polyubiquitin chains. The LotA OTU1 domain itself cleaves K6-linked ubiquitin chains, whereas it is also essential for assisting the cleavage of longer K48-linked polyubiquitin chains by the OTU2 domain. Thus, this study provides novel insights into the structure and mechanism of action of Lot DUBs. | - |
dc.language | 영어 | - |
dc.publisher | Life Science Alliance LLC | - |
dc.title | Structural insights into ubiquitin chain cleavage by Legionella ovarian tumor deubiquitinases | - |
dc.type | Article | - |
dc.identifier.doi | 10.26508/lsa.202201876 | - |
dc.citation.journaltitle | Life Science Alliance | - |
dc.identifier.wosid | 000984483500001 | - |
dc.identifier.scopusid | 2-s2.0-85153921988 | - |
dc.citation.number | 7 | - |
dc.citation.volume | 6 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Roh, Soung-Hun | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | MOLECULAR-BASIS | - |
dc.subject.keywordPlus | SPECIFICITY | - |
dc.subject.keywordPlus | CYSTEINE | - |
dc.subject.keywordPlus | DISTINCT | - |
dc.subject.keywordPlus | FAMILY | - |
dc.subject.keywordPlus | LIGASE | - |
dc.subject.keywordPlus | PROBES | - |
dc.subject.keywordPlus | DOMAIN | - |
dc.subject.keywordPlus | EXPLOITATION | - |
dc.subject.keywordPlus | DIUBIQUITIN | - |
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