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Mapping the catalytic conformations of an assembly-line polyketide synthase module
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cogan, Dillon P. | - |
dc.contributor.author | Zhang, Kaiming | - |
dc.contributor.author | Li, Xiuyuan | - |
dc.contributor.author | Li, Shanshan | - |
dc.contributor.author | Pintilie, Grigore D. | - |
dc.contributor.author | Roh, Soung-Hun | - |
dc.contributor.author | Craik, Charles S. | - |
dc.contributor.author | Chiu, Wah | - |
dc.contributor.author | Khosla, Chaitan | - |
dc.date.accessioned | 2024-05-16T01:50:54Z | - |
dc.date.available | 2024-05-16T01:50:54Z | - |
dc.date.created | 2021-11-22 | - |
dc.date.created | 2021-11-22 | - |
dc.date.issued | 2021-11 | - |
dc.identifier.citation | Science, Vol.374 No.6568, pp.729-734 | - |
dc.identifier.issn | 0036-8075 | - |
dc.identifier.uri | https://hdl.handle.net/10371/202913 | - |
dc.description.abstract | Assembly-line polyketide synthases, such as the 6-deoxyerythronolide B synthase (DEBS), are large enzyme factories prized for their ability to produce specific and complex polyketide products. By channeling protein-tethered substrates across multiple active sites in a defined linear sequence, these enzymes facilitate programmed small-molecule syntheses that could theoretically be harnessed to access countless polyketide product structures. Using cryogenic electron microscopy to study DEBS module 1, we present a structural model describing this substrate-channeling phenomenon. Our 3.2- to 4.3-angstrom-resolution structures of the intact module reveal key domain-domain interfaces and highlight an unexpected module asymmetry. We also present the structure of a product-bound module that shines light on a recently described "turnstile" mechanism for transient gating of active sites along the assembly line. | - |
dc.language | 영어 | - |
dc.publisher | American Association for the Advancement of Science | - |
dc.title | Mapping the catalytic conformations of an assembly-line polyketide synthase module | - |
dc.type | Article | - |
dc.identifier.doi | 10.1126/science.abi8358 | - |
dc.citation.journaltitle | Science | - |
dc.identifier.wosid | 000714952000032 | - |
dc.identifier.scopusid | 2-s2.0-85118893710 | - |
dc.citation.endpage | 734 | - |
dc.citation.number | 6568 | - |
dc.citation.startpage | 729 | - |
dc.citation.volume | 374 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Roh, Soung-Hun | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | CRYO-EM | - |
dc.subject.keywordPlus | EVOLUTIONARY CONSERVATION | - |
dc.subject.keywordPlus | RAPID IDENTIFICATION | - |
dc.subject.keywordPlus | EXTENDER UNIT | - |
dc.subject.keywordPlus | BIOSYNTHESIS | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordPlus | DOMAINS | - |
dc.subject.keywordPlus | ELECTROSTATICS | - |
dc.subject.keywordPlus | KETOSYNTHASE | - |
dc.subject.keywordPlus | MICROSCOPY | - |
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