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IRF8 Regulates Gram-Negative Bacteria–Mediated NLRP3 Inflammasome Activation and Cell Death : IRF8 Regulates Gram-Negative Bacteria-Mediated NLRP3 Inflammasome Activation and Cell Death
DC Field | Value | Language |
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dc.contributor.author | Karki, Rajendra | - |
dc.contributor.author | Lee, Ein | - |
dc.contributor.author | Sharma, Bhesh R. | - |
dc.contributor.author | Banoth, Balaji | - |
dc.contributor.author | Kanneganti, Thirumala-Devi | - |
dc.date.accessioned | 2024-05-16T01:57:08Z | - |
dc.date.available | 2024-05-16T01:57:08Z | - |
dc.date.created | 2023-05-08 | - |
dc.date.created | 2023-05-08 | - |
dc.date.created | 2023-05-08 | - |
dc.date.issued | 2020-05 | - |
dc.identifier.citation | Journal of Immunology, Vol.204 No.9, pp.2514-2522 | - |
dc.identifier.issn | 0022-1767 | - |
dc.identifier.uri | https://hdl.handle.net/10371/203014 | - |
dc.description.abstract | Inflammasomes are intracellular signaling complexes that are assembled in response to a variety of pathogenic or physiologic stimuli to initiate inflammatory responses. Ubiquitously present LPS in Gram-negative bacteria induces NLRP3 inflammasome activation that requires caspase-11. We have recently demonstrated that IFN regulatory factor (IRF) 8 was dispensable for caspase-11-mediated NLRP3 inflammasome activation during LPS transfection; however, its role in Gram-negative bacteria-mediated NLRP3 inflammasome activation remains unknown. In this study, we found that IRF8 promotes NLRP3 inflammasome activation in murine bone marrow-derived macrophages (BMDMs) infected with Gram-negative bacteria such as Citrobacter rodentium, Escherichia coli, or Pseudomonas aeruginosa mutant strain Delta popB. Moreover, BMDMs deficient in IRF8 showed substantially reduced caspase-11 activation and gasdermin D cleavage, which are required for NLRP3 inflammasome activation. Mechanistically, IRF8-mediated phosphorylation of IRF3 was required for Ifnb transcription, which in turn triggered the caspase-11-dependent NLRP3 inflammasome activation in the infected BMDMs. Overall, our findings suggest that IRF8 promotes NLRP3 inflammasome activation during infection with Gram-negative bacteria. | - |
dc.language | 영어 | - |
dc.publisher | American Association of Immunologists | - |
dc.title | IRF8 Regulates Gram-Negative Bacteria–Mediated NLRP3 Inflammasome Activation and Cell Death | - |
dc.title.alternative | IRF8 Regulates Gram-Negative Bacteria-Mediated NLRP3 Inflammasome Activation and Cell Death | - |
dc.type | Article | - |
dc.identifier.doi | 10.4049/jimmunol.1901508 | - |
dc.citation.journaltitle | Journal of Immunology | - |
dc.identifier.wosid | 000528255900019 | - |
dc.identifier.scopusid | 2-s2.0-85084120590 | - |
dc.citation.endpage | 2522 | - |
dc.citation.number | 9 | - |
dc.citation.startpage | 2514 | - |
dc.citation.volume | 204 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Karki, Rajendra | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | GASDERMIN D | - |
dc.subject.keywordPlus | KAPPA-B | - |
dc.subject.keywordPlus | PYROPTOSIS | - |
dc.subject.keywordPlus | TRANSCRIPTION | - |
dc.subject.keywordPlus | INFECTION | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | AIM2 | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | COMPONENTS | - |
dc.subject.keywordPlus | CLEAVAGE | - |
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