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T cells sense biophysical cues using lamellipodia and filopodia to optimize intraluminal path finding

Cited 22 time in Web of Science Cited 21 time in Scopus
Authors

Song, Kwang Hoon; Kwon, Keon Woo; Choi, Jong-Cheol; Jung, JaeHwang; Park, YongKeun; Suh, Kahp-Yang; Doh, Junsang

Issue Date
2014-04
Publisher
Royal Society of Chemistry
Citation
Integrative Biology (United Kingdom), Vol.6 No.4, pp.450-459
Abstract
Intraluminal crawling is considered to be important for extravasation of leukocytes in blood vessels, but biochemical/biophysical cues guiding the crawling of leukocytes have not been clearly understood. Here we provide evidence that T cells sense the topography of luminal surfaces and the nuclei of endothelial cells (ECs) using lamellipodia and filopodia, respectively, to optimize path finding during intraluminal crawling. Well-aligned EC layers or replicas of EC layers, which exhibit topography similar to that of EC layers, were fabricated, and flow was applied either parallel or perpendicular to the orientation of EC alignment. T cells crawled along the valleys of the topographical landscapes of the EC layers, while avoiding nuclei of ECs regardless of flow direction. Pharmacological inhibitor treatments revealed that sensing of topography and nuclei of EC layers was mediated by lamellipodia and filopodia, respectively. Lamellipodia or filopodia-inhibited T cells crawled significantly longer distances for extravasation than did normal T cells, indicating that sensing biophysical cues are critical for optimizing routes for extravasation.
ISSN
1757-9694
URI
https://hdl.handle.net/10371/203211
DOI
https://doi.org/10.1039/c4ib00021h
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  • College of Engineering
  • Department of Materials Science & Engineering
Research Area Ex Vivo Models, Lymphocyte Biology, Smart Biomaterials

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