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Simultaneous quantitative detection of 12 pathogens using high-resolution CE-SSCP
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shin, Gi Won | - |
dc.contributor.author | Hwang, Hee Sung | - |
dc.contributor.author | Oh, Mi-Hwa | - |
dc.contributor.author | Doh, Junsang | - |
dc.contributor.author | Jung, Gyoo Yeol | - |
dc.date.accessioned | 2024-05-17T07:40:57Z | - |
dc.date.available | 2024-05-17T07:40:57Z | - |
dc.date.created | 2024-05-17 | - |
dc.date.created | 2024-05-17 | - |
dc.date.issued | 2010-07 | - |
dc.identifier.citation | Electrophoresis, Vol.31 No.14, pp.2405-2410 | - |
dc.identifier.issn | 0173-0835 | - |
dc.identifier.uri | https://hdl.handle.net/10371/203229 | - |
dc.description.abstract | Several methods based on screening for a 16S ribosomal RNA gene marker have been developed for rapid and sensitive detection of pathogenic microorganisms. One such method, CE-based SSCP (CE-SSCP), has enormous potential because the technique can separate sequence variants using a simple procedure. However, conventional CE-SSCP systems have limited resolution and cannot separate most 16S ribosomal RNA gene-specific markers unless combined with additional modification steps. A high-resolution CE-SSCP system that uses a poly(ethyleneoxide)-poly(propyleneoxide)-poly(ethyleneoxide) triblock copolymer matrix was recently developed and shown to effectively separate highly similar PCR products. In this study, we developed a method based on a high-resolution CE-SSCP system using a poly(ethyleneoxide)-poly(propyleneoxide)poly(ethyleneoxide) triblock copolymer that is capable of simultaneous and quantitative detection of 12 clinically important pathogens. Pathogen markers were amplified by PCR using universal primers and separated by CE-SSCP; each marker peak was well separated at baseline and showed a characteristic mobility, allowing easy identification of pathogens. A series of experiments using different amounts of genomic pathogen DNA showed that the method had a limit of detection of 0.31-1.56 pg and a dynamic range of approximately 10(2). These results indicate that high-resolution CE-SSCP systems have considerable potential in the clinical diagnosis of bacteria-induced diseases. | - |
dc.language | 영어 | - |
dc.publisher | John Wiley & Sons Ltd. | - |
dc.title | Simultaneous quantitative detection of 12 pathogens using high-resolution CE-SSCP | - |
dc.type | Article | - |
dc.identifier.doi | 10.1002/elps.201000091 | - |
dc.citation.journaltitle | Electrophoresis | - |
dc.identifier.wosid | 000280709700014 | - |
dc.identifier.scopusid | 2-s2.0-77954712902 | - |
dc.citation.endpage | 2410 | - |
dc.citation.number | 14 | - |
dc.citation.startpage | 2405 | - |
dc.citation.volume | 31 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Doh, Junsang | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | CONFORMATION POLYMORPHISM ANALYSIS | - |
dc.subject.keywordPlus | REAL-TIME PCR | - |
dc.subject.keywordPlus | CAPILLARY-ELECTROPHORESIS | - |
dc.subject.keywordPlus | DNA MICROARRAY | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | DIAGNOSTICS | - |
dc.subject.keywordPlus | COPOLYMER | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | COMMUNITIES | - |
dc.subject.keywordPlus | SEPARATION | - |
dc.subject.keywordAuthor | 16S rRNA gene | - |
dc.subject.keywordAuthor | CE-SSCP | - |
dc.subject.keywordAuthor | High-resolution | - |
dc.subject.keywordAuthor | Pathogen detection | - |
dc.subject.keywordAuthor | Polymer matrix | - |
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