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Simultaneous quantitative detection of 12 pathogens using high-resolution CE-SSCP

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dc.contributor.authorShin, Gi Won-
dc.contributor.authorHwang, Hee Sung-
dc.contributor.authorOh, Mi-Hwa-
dc.contributor.authorDoh, Junsang-
dc.contributor.authorJung, Gyoo Yeol-
dc.date.accessioned2024-05-17T07:40:57Z-
dc.date.available2024-05-17T07:40:57Z-
dc.date.created2024-05-17-
dc.date.created2024-05-17-
dc.date.issued2010-07-
dc.identifier.citationElectrophoresis, Vol.31 No.14, pp.2405-2410-
dc.identifier.issn0173-0835-
dc.identifier.urihttps://hdl.handle.net/10371/203229-
dc.description.abstractSeveral methods based on screening for a 16S ribosomal RNA gene marker have been developed for rapid and sensitive detection of pathogenic microorganisms. One such method, CE-based SSCP (CE-SSCP), has enormous potential because the technique can separate sequence variants using a simple procedure. However, conventional CE-SSCP systems have limited resolution and cannot separate most 16S ribosomal RNA gene-specific markers unless combined with additional modification steps. A high-resolution CE-SSCP system that uses a poly(ethyleneoxide)-poly(propyleneoxide)-poly(ethyleneoxide) triblock copolymer matrix was recently developed and shown to effectively separate highly similar PCR products. In this study, we developed a method based on a high-resolution CE-SSCP system using a poly(ethyleneoxide)-poly(propyleneoxide)poly(ethyleneoxide) triblock copolymer that is capable of simultaneous and quantitative detection of 12 clinically important pathogens. Pathogen markers were amplified by PCR using universal primers and separated by CE-SSCP; each marker peak was well separated at baseline and showed a characteristic mobility, allowing easy identification of pathogens. A series of experiments using different amounts of genomic pathogen DNA showed that the method had a limit of detection of 0.31-1.56 pg and a dynamic range of approximately 10(2). These results indicate that high-resolution CE-SSCP systems have considerable potential in the clinical diagnosis of bacteria-induced diseases.-
dc.language영어-
dc.publisherJohn Wiley & Sons Ltd.-
dc.titleSimultaneous quantitative detection of 12 pathogens using high-resolution CE-SSCP-
dc.typeArticle-
dc.identifier.doi10.1002/elps.201000091-
dc.citation.journaltitleElectrophoresis-
dc.identifier.wosid000280709700014-
dc.identifier.scopusid2-s2.0-77954712902-
dc.citation.endpage2410-
dc.citation.number14-
dc.citation.startpage2405-
dc.citation.volume31-
dc.description.isOpenAccessN-
dc.contributor.affiliatedAuthorDoh, Junsang-
dc.type.docTypeArticle-
dc.description.journalClass1-
dc.subject.keywordPlusCONFORMATION POLYMORPHISM ANALYSIS-
dc.subject.keywordPlusREAL-TIME PCR-
dc.subject.keywordPlusCAPILLARY-ELECTROPHORESIS-
dc.subject.keywordPlusDNA MICROARRAY-
dc.subject.keywordPlusSYSTEM-
dc.subject.keywordPlusDIAGNOSTICS-
dc.subject.keywordPlusCOPOLYMER-
dc.subject.keywordPlusIDENTIFICATION-
dc.subject.keywordPlusCOMMUNITIES-
dc.subject.keywordPlusSEPARATION-
dc.subject.keywordAuthor16S rRNA gene-
dc.subject.keywordAuthorCE-SSCP-
dc.subject.keywordAuthorHigh-resolution-
dc.subject.keywordAuthorPathogen detection-
dc.subject.keywordAuthorPolymer matrix-
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