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Hybrid bright-field and hologram imaging of cell dynamics
Cited 11 time in
Web of Science
Cited 12 time in Scopus
- Authors
- Issue Date
- 2016-09
- Publisher
- Nature Publishing Group
- Citation
- Scientific Reports, Vol.6
- Abstract
- Volumetric observation is essential for understanding the details of complex biological phenomena. In this study, a bright-field microscope, which provides information on a specific 2D plane, and a holographic microscope, which provides information spread over 3D volumes, are integrated to acquire two complementary images simultaneously. The developed system was successfully applied to capture distinct T-cell adhesion dynamics on inflamed endothelial layers, including capture, rolling, crawling, transendothelial migration, and subendothelial migration.
- ISSN
- 2045-2322
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