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Hybrid bright-field and hologram imaging of cell dynamics

Cited 11 time in Web of Science Cited 12 time in Scopus
Authors

Byeon, Hyeokjun; Lee, Jaehyun; Doh, Junsang; Lee, Sang Joon

Issue Date
2016-09
Publisher
Nature Publishing Group
Citation
Scientific Reports, Vol.6
Abstract
Volumetric observation is essential for understanding the details of complex biological phenomena. In this study, a bright-field microscope, which provides information on a specific 2D plane, and a holographic microscope, which provides information spread over 3D volumes, are integrated to acquire two complementary images simultaneously. The developed system was successfully applied to capture distinct T-cell adhesion dynamics on inflamed endothelial layers, including capture, rolling, crawling, transendothelial migration, and subendothelial migration.
ISSN
2045-2322
URI
https://hdl.handle.net/10371/203476
DOI
https://doi.org/10.1038/srep33750
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  • College of Engineering
  • Department of Materials Science & Engineering
Research Area Ex Vivo Models, Lymphocyte Biology, Smart Biomaterials

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