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A designed supramolecular protein assembly with in vivo enzymatic activity
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Song, Woon Ju | - |
dc.contributor.author | Tezcan, F. Akif | - |
dc.date.accessioned | 2024-05-20T06:14:14Z | - |
dc.date.available | 2024-05-20T06:14:14Z | - |
dc.date.created | 2024-05-20 | - |
dc.date.created | 2024-05-20 | - |
dc.date.issued | 2014-12 | - |
dc.identifier.citation | Science, Vol.346 No.6216, pp.1525-1528 | - |
dc.identifier.issn | 0036-8075 | - |
dc.identifier.uri | https://hdl.handle.net/10371/203481 | - |
dc.description.abstract | The generation of new enzymatic activities has mainly relied on repurposing the interiors of preexisting protein folds because of the challenge in designing functional, three-dimensional protein structures from first principles. Here we report an artificial metallo-beta-lactamase, constructed via the self-assembly of a structurally and functionally unrelated, monomeric redox protein into a tetrameric assembly that possesses catalytic zinc sites in its interfaces. The designed metallo-beta-lactamase is functional in the Escherichia coli periplasm and enables the bacteria to survive treatment with ampicillin. In vivo screening of libraries has yielded a variant that displays a catalytic proficiency [(k(cat)/K-m)/k(uncat)] for ampicillin hydrolysis of 2.3 x 10(6) and features the emergence of a highly mobile loop near the active site, a key component of natural beta-lactamases to enable substrate interactions. | - |
dc.language | 영어 | - |
dc.publisher | American Association for the Advancement of Science | - |
dc.title | A designed supramolecular protein assembly with in vivo enzymatic activity | - |
dc.type | Article | - |
dc.identifier.doi | 10.1126/science.1259680 | - |
dc.citation.journaltitle | Science | - |
dc.identifier.wosid | 000346536500067 | - |
dc.identifier.scopusid | 2-s2.0-84919425588 | - |
dc.citation.endpage | 1528 | - |
dc.citation.number | 6216 | - |
dc.citation.startpage | 1525 | - |
dc.citation.volume | 346 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Song, Woon Ju | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | METALLO-BETA-LACTAMASE | - |
dc.subject.keywordPlus | EVOLUTION | - |
dc.subject.keywordPlus | ENZYMES | - |
dc.subject.keywordPlus | CATALYSIS | - |
dc.subject.keywordPlus | RESISTANCE | - |
dc.subject.keywordPlus | STABILITY | - |
dc.subject.keywordPlus | MODEL | - |
dc.subject.keywordPlus | FOLD | - |
dc.subject.keywordPlus | LOOP | - |
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