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Characterization of a Peroxodiiron(III) Intermediate in the T201S Variant of Toluene/<i>o</i>-Xylene Monooxygenase Hydroxylase from <i>Pseudomonas</i> sp OX1
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Cited 33 time in Scopus
- Authors
- Issue Date
- 2009-05
- Publisher
- AMER CHEMICAL SOC
- Citation
- JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Vol.131 No.17, pp.6074-+
- Abstract
- We report the observation of a novel intermediate in the reaction of a reduced toluene/o-xylene monooxygenase hydroxylase (ToMOH(red)) T201S variant, in the presence of a regulatory protein (ToMOD), with dioxygen. This species is the first oxygenated intermediate with an optical band in any toluene monooxygenase. The UV-vis and Mossbauer spectroscopic properties of the intermediate allow us to assign it as a peroxodiiron(III) species, T201S(peroxo), similar to H-peroxo in methane monooxygenase. Although T201S generates T201S(peroxo) in addition to optically transparent ToMOH(peroxo), previously observed in wild-type ToMOH, this conservative variant is catalytically active in steady-state catalysis and single-turnover experiments and displays the same regiospecificity for toluene and slightly different regiospecificity for o-xylene oxidation.
- ISSN
- 0002-7863
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