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Immunological synapse arrays: Patterned protein surfaces that modulate immunological synapse structure formation in T cells
DC Field | Value | Language |
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dc.contributor.author | Doh, J | - |
dc.contributor.author | Irvine, DJ | - |
dc.date.accessioned | 2024-05-20T06:15:29Z | - |
dc.date.available | 2024-05-20T06:15:29Z | - |
dc.date.created | 2024-05-17 | - |
dc.date.issued | 2006-04 | - |
dc.identifier.citation | Proceedings of the National Academy of Sciences of the United States of America, Vol.103 No.15, pp.5700-5705 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | https://hdl.handle.net/10371/203501 | - |
dc.description.abstract | T cells are activated by recognition of foreign peptides displayed on the surface of antigen presenting cells (APCs), an event that triggers assembly of a complex microscale structure at the T cell-APC interface known as the immunological synapse (IS). It remains unresolved whether the unique physical structure of the synapse itself impacts the functional response of T cells, independent of the quantity and quality of ligands encountered by the T cell. As a first step toward addressing this question, we created multicomponent protein surfaces presenting lithographically defined patterns of tethered T cell receptor (TCR) ligands (anti-CD3 "activation sites") surrounded by a field of tethered intercellular adhesion molecule-1 (ICAM-1), as a model substrate on which T cells could be seeded to mimic T cell-APC interactions. CD4(+) T cells seeded on these surfaces polarized and migrated; on contact with activation sites, T cells assembled an IS with a structure modulated by the physical pattern of ligand encountered. On surfaces patterned with focal spots of TCR ligand, T cells stably interacted with activation sites, proliferated, and secreted cytokines. In contrast, T cells interacting with activation sites patterned to preclude centralized clustering of TCR ligand failed to form stable contacts with activation sites, exhibited aberrant PKC-theta clustering in a fraction of cells, and had significantly reduced production of IFN-gamma. These results suggest that focal clustering of TCR ligand characteristic of the "mature" IS may be required under some conditions for full T cell activation. | - |
dc.language | 영어 | - |
dc.publisher | National Academy of Sciences | - |
dc.title | Immunological synapse arrays: Patterned protein surfaces that modulate immunological synapse structure formation in T cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.1073/pnas.0509404103 | - |
dc.citation.journaltitle | Proceedings of the National Academy of Sciences of the United States of America | - |
dc.identifier.wosid | 000236896200014 | - |
dc.identifier.scopusid | 2-s2.0-33645837217 | - |
dc.citation.endpage | 5705 | - |
dc.citation.number | 15 | - |
dc.citation.startpage | 5700 | - |
dc.citation.volume | 103 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Doh, J | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | ADHESION MOLECULE-1 | - |
dc.subject.keywordPlus | MONOCLONAL-ANTIBODY | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | CALCIUM | - |
dc.subject.keywordPlus | RECOGNITION | - |
dc.subject.keywordPlus | LYMPHOCYTES | - |
dc.subject.keywordPlus | DIVERSITY | - |
dc.subject.keywordPlus | BILAYERS | - |
dc.subject.keywordPlus | CD3-ZETA | - |
dc.subject.keywordPlus | SHAPE | - |
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