Publications

Detailed Information

Structural atlas of human primary microRNAs generated by SHAPE-MaP

Cited 0 time in Web of Science Cited 0 time in Scopus
Authors

Baek, S. Chan; Kim, Boseon; Jang, Harim; Kim, Kijun; Park, Il-Soo; Min, Dal-Hee; Kim, V. Narry

Issue Date
2024-03
Publisher
Cell Press
Citation
Molecular Cell, Vol.84 No.6, pp.1158-1172.e6
Abstract
MicroRNA (miRNA) maturation is critically dependent on structural features of primary transcripts (pri-miRNAs). However, the scarcity of determined pri-miRNA structures has limited our understanding of miRNA maturation. Here, we employed selective 2 ' -hydroxyl acylation analyzed by primer extension and mutational profiling (SHAPE-MaP), a high -throughput RNA structure probing method, to unravel the secondary structures of 476 high -confidence human pri-miRNAs. Our SHAPE -based structures diverge substantially from those inferred solely from computation, particularly in the apical loop and basal segments, underlining the need for experimental data in RNA structure prediction. By comparing the structures with high -throughput processing data, we determined the optimal structural features of pri-miRNAs. The sequence determinants are influenced substantially by their structural contexts. Moreover, we identified an element termed the bulged GWG motif (bGWG) with a 3 ' bulge in the lower stem, which promotes processing. Our structure -function mapping better annotates the determinants of pri-miRNA processing and offers practical implications for designing small hairpin RNAs and predicting the impacts of miRNA mutations.
ISSN
1097-2765
URI
https://hdl.handle.net/10371/203592
DOI
https://doi.org/10.1016/j.molcel.2024.02.005
Files in This Item:
There are no files associated with this item.
Appears in Collections:

Related Researcher

  • College of Natural Sciences
  • School of Biological Sciences
Research Area Molecular Biology & Genetics

Altmetrics

Item View & Download Count

  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Share