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Effects of Butylparaben Supplementation on In Vitro Development of Mouse Preantral Follicle
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Jee Hyun | - |
dc.contributor.author | Jee, Byung Chul | - |
dc.date.accessioned | 2024-05-23T04:45:33Z | - |
dc.date.available | 2024-05-23T04:45:33Z | - |
dc.date.created | 2024-05-23 | - |
dc.date.created | 2024-05-23 | - |
dc.date.created | 2024-05-23 | - |
dc.date.issued | 2020-06 | - |
dc.identifier.citation | Reproductive Sciences, Vol.27 No.6, pp.1365-1371 | - |
dc.identifier.issn | 1933-7191 | - |
dc.identifier.uri | https://hdl.handle.net/10371/203596 | - |
dc.description.abstract | We investigated whether butylparaben supplementation to the culture media negatively effects on in vitro development of mouse preantral follicle. The preantral follicles were isolated from the ovaries of 7–8-week-old mice and cultured in growth medium for 10 days and then in maturation medium for 2 days. During in vitro culture, butylparaben (0, 0.01, 0.1, 1.0, or 10 μM) was supplemented to the culture media. In the final spent media, the levels of 17ß-estradiol and anti-Müllerian hormone (AMH) were measured via enzyme-linked immunosorbent assay. In the final luteinized follicular cells, the mRNA levels of steroidogenic acute regulatory protein (StAR), superoxide dismutase 1 (Sod1), caspase 3 (Casp3), and extracellular signal-regulated kinase 1 (Erk1) were quantified via real-time reverse transcription-polymerase chain reaction. The metaphase II oocyte acquisition (per total oocyte) tended to decrease in the four butylparaben-supplemented groups, but not significant (26.8%, 23.2%, 21.4%, 15.1%, and 16.8%, respectively). The level of 17ß-estradiol and AMH tended to decrease in all butylparaben-supplemented groups, but statistically not significant. The expression level of StAR and Erk1 mRNA was significantly higher in all four butylparaben-supplemented groups, and a dose-dependent increment tendency was observed. Our findings suggest that butylparaben supplementation has largely no impact on in vitro development of mouse preantral follicle as well as 17ß-estradiol and AMH production. However, StAR, Sod1, Casp3, and Erk1 genes could be overexpressed in a certain concentration of butylparaben. | - |
dc.language | 영어 | - |
dc.publisher | Springer | - |
dc.title | Effects of Butylparaben Supplementation on In Vitro Development of Mouse Preantral Follicle | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/s43032-020-00159-w | - |
dc.citation.journaltitle | Reproductive Sciences | - |
dc.identifier.wosid | 000525431900003 | - |
dc.identifier.scopusid | 2-s2.0-85079658093 | - |
dc.citation.endpage | 1371 | - |
dc.citation.number | 6 | - |
dc.citation.startpage | 1365 | - |
dc.citation.volume | 27 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Jee, Byung Chul | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | ESTROGENIC ACTIVITY | - |
dc.subject.keywordPlus | UNITED-STATES | - |
dc.subject.keywordPlus | PARABENS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | EXPOSURE | - |
dc.subject.keywordPlus | STEROIDOGENESIS | - |
dc.subject.keywordPlus | ASSOCIATION | - |
dc.subject.keywordPlus | CHEMICALS | - |
dc.subject.keywordPlus | PRODUCTS | - |
dc.subject.keywordAuthor | Butylparaben | - |
dc.subject.keywordAuthor | Caspase | - |
dc.subject.keywordAuthor | Extracellular signal-regulated kinase | - |
dc.subject.keywordAuthor | Preantral follicle | - |
dc.subject.keywordAuthor | Steroidogenic acute regulatory protein | - |
dc.subject.keywordAuthor | Superoxide dismutase | - |
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