Publications

Detailed Information

Clinical Assessment of Intravenous Endothelial Progenitor Cell Transplantation in Dogs

Cited 5 time in Web of Science Cited 6 time in Scopus
Authors

Lee, Seok Hee; Ra, Jeong Chan; Oh, Hyun Ju; Kim, Min Jung; Setyawan, Erif Maha Nugraha; Choi, Yoo Bin; Yang, Jung Won; Kang, Sung Keun; Han, Seung Hyup; Kim, Geon A.; Lee, Byeong Chun

Issue Date
2019-07
Publisher
Cognizant Communication Corp.
Citation
Cell Transplantation, Vol.28 No.7, pp.943-954
Abstract
Endothelial progenitor cells (EPCs) have been applied for cell therapy because of their roles in angiogenesis and neovascularization in ischemic tissue. However, adverse responses caused by EPC therapy have not been fully investigated. In this study, a human peripheral blood sample was collected from a healthy donor and peripheral blood mononuclear cells were separated using Ficoll-Hypaque. There were four experimental groups: 10 ml saline infusion group (injection rate; 3 ml/min), 10 ml saline bolus group (injection rate; 60 ml/min), 10 ml EPCs infusion group (2 x 10(5) cells/ml, injection rate; 3 ml/min), 10 ml EPCs bolus group (2 x 10(5) cells/ml, injection rate; 60 ml/min). Clinical assessment included physical examination and laboratory examination for intravenous human EPC transplantation in dogs. The results revealed no remarkable findings in vital signs among the dogs used. In blood analysis, platelet counts in saline infusion groups were significantly higher than in the EPC groups within normal ranges, and no significant differences were observed except K+, Cl- and blood urea nitrogen/urea. In ELISA assay, no significant difference was observed in serum tumor necrosis factor alpha. The serum concentration of vascular endothelial growth factor was significantly higher in EPC groups than in saline groups, and interleukin 10 was significantly up-regulated in the EPC infusion group compared with other groups. In conclusion, we demonstrated that no clinical abnormalities were detected after intravenous transplantation of human EPCs in dogs. The transplanted xenogenic EPCs might be involved in anti-inflammatory and angiogenic functions in dogs.
ISSN
0963-6897
URI
https://hdl.handle.net/10371/203773
DOI
https://doi.org/10.1177/0963689718821686
Files in This Item:
There are no files associated with this item.
Appears in Collections:

Altmetrics

Item View & Download Count

  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Share