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Selenocysteine tRNA[Ser]Sec, the central component of selenoprotein biosynthesis: Isolation, identification, modification, and sequencing
DC Field | Value | Language |
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dc.contributor.author | Carlson, Bradley A. | - |
dc.contributor.author | Lee, Byeong Jae | - |
dc.contributor.author | Tsuji, Petra A. | - |
dc.contributor.author | Copeland, Paul R. | - |
dc.contributor.author | Schweizer, Ulrich | - |
dc.contributor.author | Gladyshev, Vadim N. | - |
dc.contributor.author | Hatfield, Dolph L. | - |
dc.date.accessioned | 2024-05-27T08:48:25Z | - |
dc.date.available | 2024-05-27T08:48:25Z | - |
dc.date.created | 2020-10-26 | - |
dc.date.issued | 2018-01 | - |
dc.identifier.citation | Methods in molecular biology (Clifton, N.J.), Vol.1661, pp.43-60 | - |
dc.identifier.issn | 1064-3745 | - |
dc.identifier.uri | https://hdl.handle.net/10371/203819 | - |
dc.description.abstract | © 2018, Springer Science+Business Media LLC.The selenocysteine (Sec) tRNA[Ser]Sec population consists of two isoforms that differ from each other by a single 2′-O-methylribosyl moiety at position 34 (Um34). These two isoforms, which are encoded in a single gene, Trsp, and modified posttranscriptionally, are involved individually in the synthesis of two subclasses of selenoproteins, designated housekeeping and stress-related selenoproteins. Techniques used in obtaining these isoforms for their characterization include extraction of RNA from mammalian cells and tissues, purifying the tRNA[Ser]Sec population by one or more procedures, and finally resolving the two isoforms from each other. Since some of the older techniques for isolating tRNA[Ser]Sec and resolving the isoforms are used in only a few laboratories, these procedures will be discussed briefly and references provided for more detailed information, while the more recently developed procedures are discussed in detail. In addition, a novel technique that was developed in sequencing tRNA[Ser]Sec for identifying their occurrence in other organisms is also presented. | - |
dc.language | 영어 | - |
dc.publisher | Humana Press, Inc. | - |
dc.title | Selenocysteine tRNA[Ser]Sec, the central component of selenoprotein biosynthesis: Isolation, identification, modification, and sequencing | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/978-1-4939-7258-6_4 | - |
dc.citation.journaltitle | Methods in molecular biology (Clifton, N.J.) | - |
dc.identifier.scopusid | 2-s2.0-85029600081 | - |
dc.citation.endpage | 60 | - |
dc.citation.startpage | 43 | - |
dc.citation.volume | 1661 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Lee, Byeong Jae | - |
dc.type.docType | Chapter | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordAuthor | Chromatography | - |
dc.subject.keywordAuthor | Gel electrophoresis | - |
dc.subject.keywordAuthor | Selenium | - |
dc.subject.keywordAuthor | Selenocysteine tRNA | - |
dc.subject.keywordAuthor | Selenocysteine tRNA detection | - |
dc.subject.keywordAuthor | Selenocysteine tRNA gene modification | - |
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