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Mouse models of lung-specific SARS-CoV-2 infection with moderate pathological traits
DC Field | Value | Language |
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dc.contributor.author | Kim, Sung-Hee | - |
dc.contributor.author | Kim, Jiseon | - |
dc.contributor.author | Jang, Ji Yun | - |
dc.contributor.author | Noh, Hyuna | - |
dc.contributor.author | Park, Jisun | - |
dc.contributor.author | Jeong, Haengdueng | - |
dc.contributor.author | Jeon, Donghun | - |
dc.contributor.author | Uhm, Chanyang | - |
dc.contributor.author | Oh, Heeju | - |
dc.contributor.author | Cho, Kyungrae | - |
dc.contributor.author | Jeon, Yoon | - |
dc.contributor.author | On, Dain | - |
dc.contributor.author | Yoon, Suhyeon | - |
dc.contributor.author | Lim, Soo-Yeon | - |
dc.contributor.author | Kim, Sol Pin | - |
dc.contributor.author | Lee, Youn Woo | - |
dc.contributor.author | Jang, Hui Jeong | - |
dc.contributor.author | Park, In Ho | - |
dc.contributor.author | Oh, Jooyeon | - |
dc.contributor.author | Seo, Jung Seon | - |
dc.contributor.author | Kim, Jeong Jin | - |
dc.contributor.author | Seok, Sang-Hyuk | - |
dc.contributor.author | Lee, Yu Jin | - |
dc.contributor.author | Hong, Seung-Min | - |
dc.contributor.author | An, Se-Hee | - |
dc.contributor.author | Kim, Seo Yeon | - |
dc.contributor.author | Kim, Young Been | - |
dc.contributor.author | Hwang, Ji-Yeon | - |
dc.contributor.author | Lee, Hyo-Jung | - |
dc.contributor.author | Bin Kim, Hong | - |
dc.contributor.author | Choi, Kang-Seuk | - |
dc.contributor.author | Park, Jun Won | - |
dc.contributor.author | Seo, Jun-Young | - |
dc.contributor.author | Yun, Jun-Won | - |
dc.contributor.author | Shin, Jeon-Soo | - |
dc.contributor.author | Lee, Ho-Young | - |
dc.contributor.author | Kim, Kyoungmi | - |
dc.contributor.author | Lee, Daekee | - |
dc.contributor.author | Lee, Ho | - |
dc.contributor.author | Nam, Ki Taek | - |
dc.contributor.author | Seong, Je Kyung | - |
dc.date.accessioned | 2024-08-08T01:17:45Z | - |
dc.date.available | 2024-08-08T01:17:45Z | - |
dc.date.created | 2023-03-17 | - |
dc.date.created | 2023-03-17 | - |
dc.date.issued | 2022-11 | - |
dc.identifier.citation | Frontiers in Immunology, Vol.13, p. 1055811 | - |
dc.identifier.issn | 1664-3224 | - |
dc.identifier.uri | https://hdl.handle.net/10371/205008 | - |
dc.description.abstract | Severe acute respiratory syndrome coronavirus 2 (SARS- CoV-2) causing coronavirus disease 2019 (COVID-19) has been a global health concern since 2019. The viral spike protein infects the host by binding to angiotensinconverting enzyme 2 (ACE2) expressed on the cell surface, which is then processed by type II transmembrane serine protease. However, ACE2 does not react to SARS-CoV-2 in inbred wild-type mice, which poses a challenge for preclinical research with animal models, necessitating a human ACE2 (hACE2)expressing transgenic mouse model. Cytokeratin 18 (K18) promoter-derived hACE2 transgenic mice [B6.Cg-Tg(K18-ACE2)2Prlmn/J] are widely used for research on SARS-CoV-1, MERS-CoV, and SARS-CoV-2. However, SARS-CoV2 infection is lethal at =105 PFU and SARS-CoV-2 target cells are limited to type1 alveolar pneumocytes in K18-hACE2 mice, making this model incompatible with infections in the human lung. Hence, we developed lung-specific SARSCoV-2 infection mouse models with surfactant protein B ( SFTPB) and secretoglobin family 1a member 1 (Scgb1a1) promoters. After inoculation of 105 PFU of SARS-CoV-2 to the K18-hACE2, SFTPB-hACE2, and SCGB1A1hACE2 models, the peak viral titer was detected at 2 days post-infection and then gradually decreased. In K18- hACE2 mice, the body temperature decreased by approximately 10 degrees C, body weight decreased by over 20%, and the survival rate was reduced. However, SFTPB-hACE2 and SCGB1A1-hACE2 mice showed minimal clinical signs after infection. The virus targeted type I pneumocytes in K18-hACE2 mice; type II pneumocytes in SFTPB-hACE2 mice; and club, goblet, and ciliated cells in SCGB1A1-hACE2 mice. A time-dependent increase in severe lung lesions was detected in K18-hACE2 mice, whereas mild lesions developed in SFTPB-hACE2 and SCGB1A1-hACE2 mice. Spleen, small intestine, and brain lesions developed in K18-hACE2 mice but not in SFTPBhACE2 and SCGB1A1-hACE2 mice. These newly developed SFTPB-hACE2 and SCGB1A1-hACE2 mice should prove useful to expand research on hACE2mediated respiratory viruses. | - |
dc.language | 영어 | - |
dc.publisher | Frontiers Media S.A. | - |
dc.title | Mouse models of lung-specific SARS-CoV-2 infection with moderate pathological traits | - |
dc.type | Article | - |
dc.identifier.doi | 10.3389/fimmu.2022.1055811 | - |
dc.citation.journaltitle | Frontiers in Immunology | - |
dc.identifier.wosid | 000930437900001 | - |
dc.identifier.scopusid | 2-s2.0-85143154037 | - |
dc.citation.startpage | 1055811 | - |
dc.citation.volume | 13 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Choi, Kang-Seuk | - |
dc.contributor.affiliatedAuthor | Yun, Jun-Won | - |
dc.contributor.affiliatedAuthor | Lee, Ho-Young | - |
dc.contributor.affiliatedAuthor | Seong, Je Kyung | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordAuthor | SARS-CoV-2 | - |
dc.subject.keywordAuthor | hACE2 transgenic mice | - |
dc.subject.keywordAuthor | K18-hACE2 mice model | - |
dc.subject.keywordAuthor | SFTPB-hACE2 mice model | - |
dc.subject.keywordAuthor | SCGB1A1-hACE2 mice model | - |
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