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Determination of specific interactions between glucose ligand carrying polymer and glucose transporter type-1 (GLUT-1) using different cell types

Cited 5 time in Web of Science Cited 5 time in Scopus
Authors
Chung, Jong-Hoon; Park, Keun-Hong; Seo, Byong-Moo; Kim, Eun-Seok; Hong, Jong-Rak; Chung, Il-Hyuk; Kang, Nara; Baek, Jeong-Hwa; Min, Byung-Moo; Choung, Yun-Hoon; Akaike, Toshihiro; Choung, Phill-Houng
Issue Date
2003-10
Publisher
Wiley-Blackwell
Citation
J Biomed Mater Res. 2003, 67A(3):1055-1059
Keywords
fluorescence intensityglucose transporteglycopolymerHepG2 cellspecific interaction
Abstract
In order to develop a biomimetic polymer for cell recognition, poly [3-O-(4-vinylbenzyl)-D-glucose] (PVG) and different types of glucose transport (GLUT)-carrying cells, namely, HepG2 cells (GLUT-1), 3T3-L1 fibroblast cells (GLUT-1 and GLUT-4), and MIN6 cells (GLUT-2), were tested for specific interaction. To clarify the nature of interaction between PVG and the different cell types, rhodamine-B isothiocyanate (RITC)-labeled polymers were used to prove and visualize the interactions. In this study, we found that labeled polymer strongly binds to HepG2 cells. The fluorescence intensity of PVG with in the presence of HepG2 cells was found to be stronger than that of 3T3-L1 fibroblast cells (0.11 ± 0.05) and MIN6 cells, which carry GLUT-2 (0.028 ± 0.01); a confocal laser microscopic study confirmed this interaction. To confirm the specificity of the interaction mediated by GLUT, the cells were pretreated with phloretin, an inhibitor of GLUT-1, before adding RITC-labeled PVG polymer to the cell culture medium. This treatment suppressed the interaction of PVG with HepG2 cells and partially suppressed its interaction with 3T3-L1 fibroblast cells.
Language
English
URI
https://hdl.handle.net/10371/20669
DOI
https://doi.org/10.1002/jbm.a.10472
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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