Publications
Detailed Information
Ahnak stimulates BMP2-mediated adipocyte differentiation through Smad1 activation
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shin, Sunmee | - |
dc.contributor.author | Seong, Je Kyung | - |
dc.contributor.author | Bae, Yun Soo | - |
dc.date.accessioned | 2024-08-08T01:37:20Z | - |
dc.date.available | 2024-08-08T01:37:20Z | - |
dc.date.created | 2018-08-21 | - |
dc.date.created | 2018-08-21 | - |
dc.date.issued | 2016-02 | - |
dc.identifier.citation | Obesity, Vol.24 No.2, pp.398-407 | - |
dc.identifier.issn | 1930-7381 | - |
dc.identifier.uri | https://hdl.handle.net/10371/207011 | - |
dc.description.abstract | ObjectivePrevious reports have indicated that Ahnak-deficient mice were protected from high-fat diet-induced obesity. However, the molecular mechanism in which Ahnak mediates adipocyte differentiation and high-fat diet-induced obesity is unclear. MethodsAdipocytes from Ahnak knockout (Ahnak(-/-)) mice and knockdown of Ahnak in C3H10T1/2 were used to investigate the function of Ahnak in adipocyte differentiation. Ahnak-induced adipocyte differentiation was analyzed by Oil Red O staining. ResultsAdipocytes from Ahnak(-/-) mice were smaller than those from wild-type mice. Silencing of Ahnak in C3H10T1/2 and adipose tissue-derived mesenchymal stem cells (ADSCs) from Ahnak(-/-) mice showed severely impaired adipocyte differentiation. Down-regulation of Ahnak in C3H10T1/2 cells and ADSCs from Ahnak(-/-) mice attenuated the phosphorylation and nuclear localization of Smad1 in response to BMP2, whereas Ahnak overexpression in 3T3-L1 cells significantly increased Smad1 activation. Because PPAR is a well-known transcriptional factor in adipocyte differentiation, the PPAR expression in Ahnak-mediated adipocyte differentiation was investigated. Transfection of C3H10T1/2 cells with Ahnak siRNA resulted in reduced PPAR expression apparently through inhibited binding of Smad1 to the Smad1-binding site in the PPAR promoter. These results suggest that Ahnak regulates adipogenesis by regulating Smad1-dependent PPAR expression. ConclusionsA molecular mechanism was proposed in which Ahnak regulates adipocyte differentiation through Smad1 activation. | - |
dc.language | 영어 | - |
dc.publisher | Wiley-Blackwell | - |
dc.title | Ahnak stimulates BMP2-mediated adipocyte differentiation through Smad1 activation | - |
dc.type | Article | - |
dc.identifier.doi | 10.1002/oby.21367 | - |
dc.citation.journaltitle | Obesity | - |
dc.identifier.wosid | 000369139500019 | - |
dc.identifier.scopusid | 2-s2.0-84956590421 | - |
dc.citation.endpage | 407 | - |
dc.citation.number | 2 | - |
dc.citation.startpage | 398 | - |
dc.citation.volume | 24 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Seong, Je Kyung | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | PROTEIN-KINASE-C | - |
dc.subject.keywordPlus | MESENCHYMAL PRECURSOR CELLS | - |
dc.subject.keywordPlus | SIGNALING PATHWAY | - |
dc.subject.keywordPlus | RECEPTOR-GAMMA | - |
dc.subject.keywordPlus | PPAR-GAMMA | - |
dc.subject.keywordPlus | ADIPOGENESIS | - |
dc.subject.keywordPlus | ROLES | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | PKC | - |
- Appears in Collections:
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.