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AMPK-activated protein kinase activation by Impatiens balsamina L. is related to apoptosis in HSC-2 human oral cancer cells

Cited 9 time in Web of Science Cited 10 time in Scopus
Authors

Shin, Ji-Ae; Kwon, Ki Han; Cho, Sung-Dae

Issue Date
2015-01
Publisher
Medknow Publications
Citation
Pharmacognosy Magazine, Vol.11 No.41, pp.136-142
Abstract
Objective: In the present study, we investigated the efficacy of a methanol extract from Impatiens balsamina L. (MEIB) against HSC-2 human oral cancer cells. Materials and Methods: The anti-cancer efficacies of MEIB were performed by methanethiosulfonate assay, phospho-kinase array, Western blot, 4- 6-diamidino-2-phenylindole staining, trypan blue exclusion assay and 5,5,6,6-tetrachloro-1,1,3,3- tetraethylbenzimidazolylcarbocyanine iodide assay. Results: MEIB decreased the cell viability of HSC-2 cells. According to phospho-kinase arrays, MEIB markedly activated AMP-activated protein kinase (AMPK) signaling, but inactivated mammalian target of rapamycin signaling. MEIB induced apoptosis as evidenced by activation of caspase-3, poly (ADP-ribose) polymerase cleavage and nuclear condensation. In addition, AMPK activation by two known activators (5-aminoimidazole-4-carboxamide-1--ribofuranoside and metformin) decreased cell viability and induced apoptosis. Moreover, MEIB increased the expression levels of mitochondria-related proteins (t-Bid, Bak and Bad), which contributed to the disruption of mitochondrial membrane potential, cytochrome C release and activation of caspase-9. Metformin also increased t-Bid expression and the subsequent release of cytochrome C into the cytosol. Conclusion: These results suggest that MEIB may be of therapeutic value for treating oral cancer and that its mechanism of action occurs through AMPK and t-Bid.
ISSN
0973-1296
URI
https://hdl.handle.net/10371/207297
DOI
https://doi.org/10.4103/0973-1296.149728
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  • School of Dentistry
  • Department of Dentistry
Research Area Discovery of molecular targets related to oral cancer metastasis and identification of signal transduction system, Identifying the role of immunological tolerance in oral cancer, Presenting a new concept oral cancer prevention and treatment strategy through identification of major molecular targets and mechanisms related to oral cancer development, 구강암 발병관련 주요 분자표적 및 기전 규명을 통한 신개념 구강암 예방 및 치료전략 제시, 구강암 전이관련 분자표적 발굴 및 신호전달체계 규명, 구강암에서 면연관용의 역할 규명

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