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Determination of phenanthrene bioavailability by using a self-dying reporter bacterium: Test with model solids and soil
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shin, Doyun | - |
dc.contributor.author | Nam, Kyoungphile | - |
dc.date.accessioned | 2024-08-08T01:45:28Z | - |
dc.date.available | 2024-08-08T01:45:28Z | - |
dc.date.created | 2020-08-26 | - |
dc.date.created | 2020-08-26 | - |
dc.date.issued | 2012-02 | - |
dc.identifier.citation | Journal of Biotechnology, Vol.157 No.4, pp.454-459 | - |
dc.identifier.issn | 0168-1656 | - |
dc.identifier.uri | https://hdl.handle.net/10371/207885 | - |
dc.description.abstract | The present study was conducted to investigate the performance and feasibility of a self-dying reporter bacterium to visualize and quantify phenanthrene bioavailability in soil. The self-dying reporter bacterium was designed to die on the initiation of phenanthrene biodegradation. The viability of the reporter bacterium was determined by a fluorescence live/dead cell staining method and visualized by confocal laser scanning microscopic observation. Phenanthrene was spiked into four types of model solids and a sandy loam. The bioavailability of phenanthrene to the reporter bacterium was remarkably declined with the hydrophobicity of the model solids: essentially no phenanthrene was biodegraded in the presence of 9-nm pores and about 35.8% of initial phenanthrene was biodegraded without pores. Decrease in bioavailability was not evident in the nonporous hydrophilic bead, but a small decrease was observed in the porous hydrophilic bead at 1000 mg/kg of phenanthrene. The fluorescence intensity was commensurate with the extent of phenanthrene biodegradation by the reporter bacterium at the concentration range from 50 to 500 mg/kg. Such a quantitative relationship was also confirmed with a sandy loam spiked up to 1000 mg/kg of phenanthrene. This reporter bacterium may be a useful means to determine phenanthrene bioavailability in soil. (C) 2011 Elsevier B.V. All rights reserved. | - |
dc.language | 영어 | - |
dc.publisher | Elsevier BV | - |
dc.title | Determination of phenanthrene bioavailability by using a self-dying reporter bacterium: Test with model solids and soil | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.jbiotec.2011.07.041 | - |
dc.citation.journaltitle | Journal of Biotechnology | - |
dc.identifier.wosid | 000301900900003 | - |
dc.identifier.scopusid | 2-s2.0-84857458187 | - |
dc.citation.endpage | 459 | - |
dc.citation.number | 4 | - |
dc.citation.startpage | 454 | - |
dc.citation.volume | 157 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Nam, Kyoungphile | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | POLYCYCLIC AROMATIC-HYDROCARBONS | - |
dc.subject.keywordPlus | BIODEGRADATION | - |
dc.subject.keywordPlus | SEDIMENTS | - |
dc.subject.keywordPlus | BIOSENSOR | - |
dc.subject.keywordPlus | TOXICITY | - |
dc.subject.keywordPlus | RISK | - |
dc.subject.keywordAuthor | Phenanthrene | - |
dc.subject.keywordAuthor | Model solid | - |
dc.subject.keywordAuthor | Soil | - |
dc.subject.keywordAuthor | Bioavailability | - |
dc.subject.keywordAuthor | Visualization | - |
dc.subject.keywordAuthor | Self-dying reporter bacterium | - |
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