전자빔을 조사한 7대 3 비율의 수산화인회석과 인산삼칼슘 혼합무기물의 골재생 효과

Abstract

The aim of this study is to evaluate the effect and potential of electron beam (E-beam) irradiation treatment to the new bone formation in the rat calvarial bony defects by using of 7 to 3 ratios of hydroxyapatite (HA, Bongros (R), Bio@Co., KOREA) and tricalcium phosphate (beta-TCP, Sigma-Aldrich Co., USA) mixed with type I collagen (Rat tail, BD Biosciences Co., Sweden). We used 1.0 MeV linear accelerator and 2.0 MeV superconductive linear accelerator (power 100 KW, pressure 115 kPa, temperature - 30 similar to 120 degrees C, sensor sensitivity 0.1 similar to 1.2 mV/kPa, generating power sensitivity 44.75 mV/kPa, supply voltage 5 +/- 0.25 V) with different irradiation dose, such as 1, 30, 60 kGy. Bilateral, standardized transosseous circular calvarial defects, 6.0 mm in diameter, were made in a total of twenty-one, 8-weeks-old, male Sprague-Dawley rats, weighing 270-300 g. In six different experimental groups, the defect of every three rats was filled with E-beam irradiated bony mixtures, which different energy and dose, and that of rats without E-beam irradiated bony mixtures can be compared with our previous results. The other three rats were used as sham group. Rats were sacrificed after 4, 8, and 16 weeks, and grafted healing specimens were studied by clinical and radiographic (standardized plain film, Kodak Co., USA) findings, histomorphologic staining with hematoxylin and eosin (H & E), FE-SEM (field-emission scanning electron microscopy), and TEM (transmission electron microscopy) findings. The large particular size of HA was changed to small particles after E-beam irradiation, to which small particle of TCP was engaged to small sixed HA with organic collagen components in SEM findings. Abundant endothelial cells with pericytes were found around inner portions of grafted healing bone and many couplings of osteoblast with osteoclast in TEM findings were found in every experimental groups.