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Use of reporter-gene based bacteria to quantify phenanthrene biodegradation and toxicity in soil
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shin, Doyun | - |
dc.contributor.author | Moon, Hee Sun | - |
dc.contributor.author | Lin, Chu-Ching | - |
dc.contributor.author | Barkay, Tamar | - |
dc.contributor.author | Nam, Kyoungphile | - |
dc.date.accessioned | 2024-08-08T01:46:27Z | - |
dc.date.available | 2024-08-08T01:46:27Z | - |
dc.date.created | 2020-08-18 | - |
dc.date.created | 2020-08-18 | - |
dc.date.issued | 2011-02 | - |
dc.identifier.citation | Environmental Pollution, Vol.159 No.2, pp.509-514 | - |
dc.identifier.issn | 0269-7491 | - |
dc.identifier.uri | https://hdl.handle.net/10371/208027 | - |
dc.description.abstract | A phenanthrene-degrading bacterium, Sphingomonas paucimobilis EPA505 was used to construct two fluorescence-based reporter strains. Strain D harboring gfp gene was constructed to generate green fluorescence when the strain started to biodegrade phenanthrene. Strain S possessing gef gene was designed to die once phenanthrene biodegradation was initiated and thus to lose green fluorescence when visualized by a live/dead cell staining. Confocal laser scanning microscopic observation followed by image analysis demonstrates that the fluorescence intensity generated by strain D increased and the intensity by strain S decreased linearly at the phenanthrene concentration of up to 200 mg/L Such quantitative increase and decrease of fluorescence intensity in strain D (i.e., from 1 to 11.90 +/- 0.72) and strain S (from 1 to 0.40 +/- 0.07) were also evident in the presence of Ottawa sand spiked with the phenanthrene up to 1000 mg/kg. The potential use of the reporter strains in quantitatively determining biodegradable or toxic phenanthrene was discussed. (C) 2010 Elsevier Ltd. All rights reserved. | - |
dc.language | 영어 | - |
dc.publisher | Pergamon Press Ltd. | - |
dc.title | Use of reporter-gene based bacteria to quantify phenanthrene biodegradation and toxicity in soil | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.envpol.2010.10.017 | - |
dc.citation.journaltitle | Environmental Pollution | - |
dc.identifier.wosid | 000286963000022 | - |
dc.identifier.scopusid | 2-s2.0-78650509901 | - |
dc.citation.endpage | 514 | - |
dc.citation.number | 2 | - |
dc.citation.startpage | 509 | - |
dc.citation.volume | 159 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Nam, Kyoungphile | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | POLYCYCLIC AROMATIC-HYDROCARBONS | - |
dc.subject.keywordPlus | BIOAVAILABLE TOLUENE | - |
dc.subject.keywordPlus | BIOSENSOR | - |
dc.subject.keywordPlus | FLUORANTHENE | - |
dc.subject.keywordPlus | NAPHTHALENE | - |
dc.subject.keywordPlus | PLASMID | - |
dc.subject.keywordPlus | BIOLUMINESCENCE | - |
dc.subject.keywordPlus | EXTRACTABILITY | - |
dc.subject.keywordPlus | CYCLODEXTRIN | - |
dc.subject.keywordPlus | DERIVATIVES | - |
dc.subject.keywordAuthor | Phenanthrene | - |
dc.subject.keywordAuthor | Bioavailability | - |
dc.subject.keywordAuthor | Toxicity | - |
dc.subject.keywordAuthor | Reporter strain | - |
dc.subject.keywordAuthor | gfp gene | - |
dc.subject.keywordAuthor | gef gene | - |
dc.subject.keywordAuthor | Confocal laser scanning microscope | - |
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