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Duplex pyrosequencing assay of the 388A>G and 521T>C SLCO1B1 polymorphisms in three Asian populations

Cited 14 time in Web of Science Cited 16 time in Scopus
Authors
Kim, Eun-Young; Cho, Doo-Yeoun; Shin, Ho-Jung; Lee, Sang-Seop; Shon, Ji-Hong; Shin, Jae-Gook; Shin, Sang-Goo
Issue Date
2008
Publisher
Elsevier
Citation
Clin Chim Acta 2008;388:68-72
Keywords
AllelesAmino Acids/geneticsAsian Continental Ancestry Group/*geneticsHaplotypes/geneticsOrganic Anion Transporters/*geneticsPolymorphism, Genetic/*geneticsSequence Analysis, DNA/*methods
Abstract
BACKGROUND: We developed a method for detecting important SLCO1B1 polymorphisms and compared the haplotype frequencies in 3 Asian populations. METHODS: We designed a duplex pyrosequencing assay to detect simultaneously the 388A>G and 521T>C variants of SLCO1B1; this method can identify SLCO1B1*1b, SLCO1B1*5, and SLCO1B1*15. The method was validated by direct sequencing of 96 Korean subjects. In addition, duplex genotyping and the monoplex method were compared and validated with 469 Korean subjects. To characterize the haplotype frequencies based on the 2 polymorphisms, we genotyped 106 Chinese and 104 Vietnamese subjects, as well as Korean subjects, using the new method. RESULTS: The results showed 100% concordance among the monoplex and duplex pyrosequencing assays and direct sequencing method. The allele frequencies were similar in the 3 Asian populations: the most common allele was SLCO1B1*1b, while SLCO1B1*5 was rare or absent. The frequencies of functional SLCO1B1*15 alleles differed statistically between Chinese (8.2%) and Korean (14.0%) and Vietnamese (16.3%) (p<0.05, chi(2)-test). CONCLUSIONS: The duplex pyrosequencing assay appears to be an accurate, rapid, and cost-effective genotyping method to detect major SLCO1B1 important alleles in Asian populations.
ISSN
0009-8981 (Print)
Language
English
URI
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T57-4PWF0V0-1&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=ad46453ea6bd32836e6e7a5695907fba

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17996736

https://hdl.handle.net/10371/22186
DOI
https://doi.org/10.1016/j.cca.2007.10.010
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College of Medicine/School of Medicine (의과대학/대학원)Program in Clinical Pharmacology (협동과정-임상약리학전공)Journal Papers (저널논문_협동과정-임상약리학전공)
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