S-Space College of Medicine/School of Medicine (의과대학/대학원) Program in Clinical Pharmacology (협동과정-임상약리학전공) Journal Papers (저널논문_협동과정-임상약리학전공)
Duplex pyrosequencing assay of the 388A>G and 521T>C SLCO1B1 polymorphisms in three Asian populations
- Kim, Eun-Young; Cho, Doo-Yeoun; Shin, Ho-Jung; Lee, Sang-Seop; Shon, Ji-Hong; Shin, Jae-Gook; Shin, Sang-Goo
- Issue Date
- Clin Chim Acta 2008;388:68-72
- Alleles; Amino Acids/genetics; Asian Continental Ancestry Group/*genetics; Haplotypes/genetics; Organic Anion Transporters/*genetics; Polymorphism, Genetic/*genetics; Sequence Analysis, DNA/*methods
- BACKGROUND: We developed a method for detecting important SLCO1B1 polymorphisms and compared the haplotype frequencies in 3 Asian populations. METHODS: We designed a duplex pyrosequencing assay to detect simultaneously the 388A>G and 521T>C variants of SLCO1B1; this method can identify SLCO1B1*1b, SLCO1B1*5, and SLCO1B1*15. The method was validated by direct sequencing of 96 Korean subjects. In addition, duplex genotyping and the monoplex method were compared and validated with 469 Korean subjects. To characterize the haplotype frequencies based on the 2 polymorphisms, we genotyped 106 Chinese and 104 Vietnamese subjects, as well as Korean subjects, using the new method. RESULTS: The results showed 100% concordance among the monoplex and duplex pyrosequencing assays and direct sequencing method. The allele frequencies were similar in the 3 Asian populations: the most common allele was SLCO1B1*1b, while SLCO1B1*5 was rare or absent. The frequencies of functional SLCO1B1*15 alleles differed statistically between Chinese (8.2%) and Korean (14.0%) and Vietnamese (16.3%) (p<0.05, chi(2)-test). CONCLUSIONS: The duplex pyrosequencing assay appears to be an accurate, rapid, and cost-effective genotyping method to detect major SLCO1B1 important alleles in Asian populations.
- 0009-8981 (Print)
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