S-Space College of Medicine/School of Medicine (의과대학/대학원) Immunology (면역학전공) Journal Papers (저널논문_면역학전공)
Stage-specific expression of two neighboring Crlz1 and IgJ genes during B cell development is regulated by their chromatin accessibility and histone acetylation
- Lim, J. H.; Cho, S. J.; Park, S. K.; Kim, J.; Cho, D.; Lee, W. J.; Kang, C. J.
- Issue Date
- American Association of Immunologists
- J Immunol. 2006 Oct 15;177(8):5420-9.
- Acetylation; Animals; B-Lymphocytes/*cytology/immunology; Cell Line; Chromatin/*metabolism; DNA-Binding Proteins/*genetics; Gene Expression Regulation/*immunology; Histones/*metabolism; Immunoglobulin J-Chains/*genetics; Mice; Nerve Tissue Proteins/*genetics; Plasma Cells/cytology/immunology; Promoter Regions, Genetic; Transcription Factors/*genetics
- The IgJ gene is expressed in the plasma cell stage. However, its neighboring charged amino acid-rich leucine zipper 1 (Crlz1) gene, which is mapped 30 kb upstream of the IgJ gene in mice, is shown to be expressed in the pre-B cell stage. These stage-specific expressions of two neighboring genes are found to be regulated by their chromatin accessibility and acetylation. Hypersensitive site 1 on the IgJ promoter is opened in the plasma cells, whereas hypersensitive sites 9/10 on the Crlz1 promoter are opened in the pre-B cells. Furthermore, H3 and H4 histones toward the chromatin of the Crlz1 gene are found to be hyperacetylated, especially on H3, in the pre-B cells, whereas those toward the chromatin of the IgJ gene are found to be hyperacetylated in the plasma cells. Consistently, the hyperacetylation of H3 and H4 toward the chromatin of the IgJ gene but not the Crlz1 gene is induced by an IL-2 treatment of BCL1, which is a model cell line for studying the terminal differentiation of B cells.
- 0022-1767 (Print)
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