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FcgammaRIII engagement provides activating signals to NKT cells in antibody-induced joint inflammation

Cited 55 time in Web of Science Cited 58 time in Scopus
Authors

Kim, H. Y.; Kim, S.; Chung, D. H.

Issue Date
2006-08-19
Publisher
American Society for Clinical Investigation
Citation
J Clin Invest. 2006 Sep;116(9):2484-92. Epub 2006 Aug 17.
Keywords
Adoptive TransferAnimalsArthritis, Experimental/*immunologyCell LineDNA PrimersImmunoglobulin G/immunologyInflammation/immunologyInterferon-gamma/biosynthesisInterleukins/biosynthesisJoint Diseases/immunologyKiller Cells, Natural/*immunologyMiceMice, Inbred NODMice, TransgenicReceptors, IgG/deficiency/genetics/*physiologyReverse Transcriptase Polymerase Chain ReactionT-Lymphocyte Subsets/*immunologyT-Lymphocytes, Regulatory/immunology
Abstract
NKT cells promote antibody-induced arthritis, but the mechanism by which NKT cells are activated in this model remains unclear. It has been proposed that Fcgamma receptor (FcgammaR) contributes to NKT cell activation in antibody-induced arthritis. To address this issue, we explored the functions of FcgammaR on NKT cells in antibody-induced arthritis. RT-PCR and flow cytometric analysis demonstrated that NKT cells constitutively express surface FcgammaRIII but not FcgammaRI, -II, or -IV. FcgammaRIII engagement by aggregated IgG on NKT cells enhanced CD25 and CD69 expression, whereas FcgammaR(-/-) mouse NKT cells did not enhance activation. FcgammaRIII engagement on NKT cells enhanced the production of IL-4, IL-10, IL-13, and IFN-gamma, whereas FcgammaR-deficient NKT cells did not alter the production of these cytokines after aggregated IgG treatment. However, FcgammaR-deficient NKT cells were functionally intact in terms of TCR-induced activation. Moreover, adoptive transfer of FcgammaR-deficient NKT cells could not restore inflammation or TGF-beta production in the joint tissues of CD1d(-/-) mice, whereas adoptive transfer of wild-type NKT cells induced arthritis and reduced TGF-beta production in joint tissues. We conclude that FcgammaRIII engagement by IgG in joint tissues provides activating signals to NKT cells in antibody-induced arthritis.
ISSN
0021-9738 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16917543

https://hdl.handle.net/10371/22586
DOI
https://doi.org/10.1172/JCI27219
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