Publications
Detailed Information
Heat shock protein 70 negatively regulates the heat-shock-induced suppression of the IkappaB/NF-kappaB cascade by facilitating IkappaB kinase renaturation and blocking its further denaturation
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, K. H. | - |
dc.contributor.author | Lee, C. T. | - |
dc.contributor.author | Kim, Y. W. | - |
dc.contributor.author | Han, S. K. | - |
dc.contributor.author | Shim, Y. S. | - |
dc.contributor.author | Yoo, C. G. | - |
dc.date.accessioned | 2009-12-24T11:22:24Z | - |
dc.date.available | 2009-12-24T11:22:24Z | - |
dc.date.issued | 2005-06-01 | - |
dc.identifier.citation | Exp Cell Res. 2005 Jul 1;307(1):276-84. Epub 2005 Apr 13. | en |
dc.identifier.issn | 0014-4827 (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15922746 | - |
dc.identifier.uri | https://hdl.handle.net/10371/22618 | - |
dc.description.abstract | Heat shock (HS) treatment has been previously shown to suppress the IkappaB/nuclear factor-kappaB (NF-kappaB) cascade by denaturing, and thus inactivating IkappaB kinase (IKK). HS is characterized by the induction of a group of heat shock proteins (HSPs). However, their role in the HS-induced suppression of the IkappaB/NF-kappaB cascade is unclear. Adenovirus-mediated HSP70 overexpression was found not to suppress the TNF-alpha-induced activation of the IkappaB/NF-kappaB pathway, thus suggesting that HSP70 is unlikely to suppress this pathway. When TNF-alpha-induced activation of the IkappaB/NF-kappaB pathway was regained 24 h after HS, HSP70 was found to be highly up-regulated. Moreover, blocking HSP70 induction delayed TNF-alpha-induced IkappaBalpha degradation and the resolubilization of IKK. In addition, HSP70 associated physically with IKK, suggesting that HSP70 is involved in the recovery process via molecular chaperone effect. Adenovirus-mediated HSP70 overexpression prior to HS blocked the IkappaBalpha stabilizing effect of HS by suppressing IKK insolubilization. Moreover, the up-regulation of endogenous HSP70 by preheating, suppressed this subsequent HS-induced IKK insolubilization, and this effect was abrogated by blocking HSP70 induction. These findings indicate that HSP70 accumulates during HS and negatively regulates the HS-induced suppression of the IkappaB/NF-kappaB cascade by facilitating the renaturation of IKK and blocking its further denaturation. | en |
dc.language.iso | en | en |
dc.publisher | Elsevier | en |
dc.subject | Adenoviridae/genetics | en |
dc.subject | Blotting, Western | en |
dc.subject | Bronchi/cytology | en |
dc.subject | Cell Culture Techniques | en |
dc.subject | Cell Line | en |
dc.subject | Electrophoretic Mobility Shift Assay | en |
dc.subject | Enzyme Activation | en |
dc.subject | Epithelial Cells/cytology | en |
dc.subject | Gene Expression Regulation/drug effects/*genetics | en |
dc.subject | HSP70 Heat-Shock Proteins/biosynthesis/metabolism | en |
dc.subject | Heat-Shock Response/drug effects/*genetics | en |
dc.subject | Hot Temperature | en |
dc.subject | Humans | en |
dc.subject | I-kappa B Proteins/*metabolism | en |
dc.subject | NF-kappa B/genetics/*metabolism/pharmacology | en |
dc.subject | Recombinant Proteins/metabolism/pharmacology | en |
dc.subject | Time Factors | en |
dc.subject | Transduction, Genetic | en |
dc.subject | Up-Regulation | en |
dc.subject | Protein Renaturation | - |
dc.title | Heat shock protein 70 negatively regulates the heat-shock-induced suppression of the IkappaB/NF-kappaB cascade by facilitating IkappaB kinase renaturation and blocking its further denaturation | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 이경희 | - |
dc.contributor.AlternativeAuthor | 이춘택 | - |
dc.contributor.AlternativeAuthor | 김영환 | - |
dc.contributor.AlternativeAuthor | 한성구 | - |
dc.contributor.AlternativeAuthor | 심영수 | - |
dc.contributor.AlternativeAuthor | 유철규 | - |
dc.identifier.doi | 10.1016/j.yexcr.2005.03.014 | - |
- Appears in Collections:
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.