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CD19 signalling improves the Epstein-Barr virus-induced immortalization of human B cell
Cited 12 time in
Web of Science
Cited 14 time in Scopus
- Authors
- Issue Date
- 2005-02-01
- Publisher
- Wiley-Blackwell
- Citation
- Cell Prolif. 2005 Feb;38(1):35-45.
- Keywords
- Antigens, CD19/*biosynthesis ; B-Lymphocytes/*cytology/metabolism ; Cell Line ; Cell Membrane/metabolism ; Cell Separation ; Cell Transformation, Viral ; Epstein-Barr Virus Nuclear Antigens/metabolism ; Flow Cytometry ; Herpesvirus 4, Human/*metabolism ; Humans ; Leukocytes, Mononuclear/metabolism ; Lymphocytes/virology ; Phenotype ; Reverse Transcriptase Polymerase Chain Reaction ; Time Factors ; Viral Matrix Proteins/metabolism ; Viral Proteins ; Signal Transduction
- Abstract
- Epstein-Barr virus (EBV) infection in vitro immortalizes primary B cells and generates B lymphoblastoid cell lines (LCLs). These EBV-LCLs have been used for several purposes in immunological and genetic studies, but some trials involving these transformations fail for unknown reasons, and several EBV-LCLs do not grow in normal culture. In this study, we improved the immortalization method by CD19 and B-cell receptor (BCR) co-ligation. This method shortens the time required for the immortalization and generation of EBV-LCLs but does not alter the cell phenotype of the LCLs nor the expression of the EBV genes. In particular, the CD19 and BCR co-ligation method was found to be the most effective method examined. EBV-infected B cells induced by CD19 and/or BCR ligation expressed the intracellular latent membrane protein LMP-1 earlier than EBV-infected B cells, and the expression of intracellular LMP-1 was found to be closely related to the time of immortalization. These results suggest that the modified method, using CD19 and/or BCR ligation, may efficiently generate EBV-LCLs, by expressing intracellular LMP-1 at an early stage.
- ISSN
- 0960-7722 (Print)
- Language
- English
- URI
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15679865
https://hdl.handle.net/10371/22639
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