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MIDGE/hNIS vaccination generates antigen-associated CD8+IFN-gamma+ T cells and enhances protective antitumor immunity

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dc.contributor.authorChoi, Yun-
dc.contributor.authorJeon, Yong-Hyun-
dc.contributor.authorKang, Joo-Hyun-
dc.contributor.authorChung, June-Key-
dc.contributor.authorSchmidt, Manuel-
dc.contributor.authorKim, And Chul-Woo-
dc.date.accessioned2009-12-28T09:03:26Z-
dc.date.available2009-12-28T09:03:26Z-
dc.date.issued2007-02-03-
dc.identifier.citationInt J Cancer. 2007 May 1;120(9):1942-50.en
dc.identifier.issn0020-7136 (Print)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17266027-
dc.identifier.urihttps://hdl.handle.net/10371/22873-
dc.description.abstractHuman sodium iodide symporter (hNIS) is a transmembrane protein that actively transports iodide ions into thyroid cells. hNIS is over-expressed in some cases of the thyroid cancers compared with the surrounding normal tissues and has been considered to be an attractive target for immunotherapy. The aim of this study is to determine the feasibility of utilizing the hNIS antigenic protein in enhanced-antigen-associated immunotherapy using image analysis with a gamma counter. To accomplish this, minimalistic immunogenically defined gene expression (MIDGE), either plain or coupled to a nuclear localization signal (NLS) peptide, was used as a vector system. Vaccination with MIDGE/hNIS, MIDGE/hNIS-NLS and pcDNA3.1/hNIS produced a significant increase in the number of hNIS-associated IFN-gamma-secreting CD8(+) T cells, with MIDGE/hNIS having the strongest effect. In addition, immunization with the hNIS encoding vectors induced antigen-mediated antitumor activity against NIS-expressing CT26 tumors in vivo, with the highest tumor free rate (100%) and lowest tumor growth being observed up to 40 days after the CT26/NIS tumor challenge with MIDGE/hNIS than those resulting from other immunization groups. Tumor progression could be followed noninvasively and repetitively by monitoring levels of hNIS gene expression in the tumors using scintigraphic image analysis. Overall, hNIS has a potential use as an antigen for immunization approaches, and vaccination with MIDGE/hNIS vectors is an effective means of generating hNIS-associated immune responses in mice.en
dc.language.isoen-
dc.publisherWiley-Blackwellen
dc.subjectAnimalsen
dc.subjectCD4-Positive T-Lymphocytes/immunologyen
dc.subjectCD8-Positive T-Lymphocytes/*immunologyen
dc.subjectCancer Vaccines/*immunologyen
dc.subjectFemaleen
dc.subject*Genetic Vectorsen
dc.subjectHumansen
dc.subjectInterferon-gamma/*biosynthesisen
dc.subjectMiceen
dc.subjectMice, Inbred BALB Cen
dc.subjectNeoplasms, Experimental/immunology/prevention & controlen
dc.subjectSymporters/genetics/*immunologyen
dc.subjectTechnetium/diagnostic useen
dc.subjectVaccinationen
dc.subjectVaccines, DNA/*immunologyen
dc.titleMIDGE/hNIS vaccination generates antigen-associated CD8+IFN-gamma+ T cells and enhances protective antitumor immunityen
dc.typeArticleen
dc.contributor.AlternativeAuthor최윤-
dc.contributor.AlternativeAuthor전용현-
dc.contributor.AlternativeAuthor강주현-
dc.contributor.AlternativeAuthor정준기-
dc.contributor.AlternativeAuthor김철우-
dc.identifier.doi10.1002/ijc.22567-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Program in Cancer Biology (협동과정-종양생물학전공)Journal Papers (저널논문_협동과정-종양생물학전공)
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