S-Space College of Medicine/School of Medicine (의과대학/대학원) Program in Cancer Biology (협동과정-종양생물학전공) Journal Papers (저널논문_협동과정-종양생물학전공)
DLC-1, a GTPase-activating protein for Rho, is associated with cell proliferation, morphology, and migration in human hepatocellular carcinoma
- Kim, Tai Young; Lee, Jung Weon; Kim, Hwang-Phill; Jong, Hyun-Soon; Kim, Tae-You; Jung, Mira; Bang, Yung-Jue
- Issue Date
- Biochem. Biophys. Res. Commun. 355 (2007) 72-77
- Carcinoma, Hepatocellular/metabolism/*pathology; Cell Division; Cell Line; Cell Line, Tumor; Cell Movement; Humans; Kidney; Liver Neoplasms/metabolism/*pathology; Mutagenesis, Site-Directed; Phosphoproteins/metabolism; Polymerase Chain Reaction; RNA, Messenger/genetics; Recombinant Proteins/metabolism; Tumor Suppressor Proteins/*genetics/metabolism
- DLC-1 (deleted in liver cancer-1) is a tumor suppressor gene for hepatocellular carcinoma and other cancers. To characterize its functions, we constructed recombinant adenovirus encoding the wild-type DLC-1 and examined its effects on behaviors of a hepatocellular carcinoma cell line (SNU-368), which does not express DLC-1. Here, we found that restoration of DLC-1 expression in the SNU-368 cells caused an inhibition of cell proliferation with an increase of a subG1 population. Furthermore, DLC-1 overexpression induced disassembly of stress fibers and extensive membrane protrusions around cells on laminin-1. DLC-1 overexpression also inhibited cell migration and dephosphorylated focal adhesion proteins such as focal adhesion kinase (FAK), Cas (p130Cas; Crk-associated substrate), and paxillin. These observations suggest that DLC-1 plays important roles in signal transduction pathway regulating cell proliferation, cell morphology, and cell migration by affecting Rho family GTPases and focal adhesion proteins.
- 0006-291X (Print)
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