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Improvement of neutralizing activity of human scFv antibodies against hepatitis B virus binding using CDR3 V(H) mutant library
DC Field | Value | Language |
---|---|---|
dc.contributor.author | PARK, SAE-GWANG | - |
dc.contributor.author | JUNG, YOUNG-JOO | - |
dc.contributor.author | LEE, YOUNG-YI | - |
dc.contributor.author | YANG, CHEUL-MIN | - |
dc.contributor.author | KIM, IK-JUNG | - |
dc.contributor.author | CHUNG, JUN-HO | - |
dc.contributor.author | KIM, IK-SANG | - |
dc.contributor.author | LEE, YOUN-JAE | - |
dc.contributor.author | PARK, SUNG-JAE | - |
dc.contributor.author | LEE, JEONG-NYEO | - |
dc.contributor.author | SEO, SU-KIL | - |
dc.contributor.author | PARK, YONG-HONG | - |
dc.contributor.author | CHOI, IN-HAK | - |
dc.date.accessioned | 2009-12-29T08:37:54Z | - |
dc.date.available | 2009-12-29T08:37:54Z | - |
dc.date.issued | 2006 | - |
dc.identifier.citation | Viral Immunol. 19:115-23 | en |
dc.identifier.issn | 0882-8245 (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16553557 | - |
dc.identifier.uri | https://hdl.handle.net/10371/23287 | - |
dc.description.abstract | CDR3 of the heavy-chain variable region of immunoglobulin is a region in which somatic mutation occurs heavily after secondary antibody response, resulting in an affinity maturation of antibodies in vivo. The aim of this study was to improve the affinity of a human single-chain variable fragment (scFv) specific for pre-S1 of hepatitis B virus (HBV) by introducing random mutagenesis in CDR3 variable region of heavy chain (V(H)) of the parental scFv clone 1E4. By using a BIAcore for panning and screening, we have selected three clones (A9, B2, and B9) with lower highest affinity (K(D)) than 1E4. Affinities of selected clones ranged from 1.7 x 10(7) mol/L to 6.3 x 10(8) mol/L, which were increased by factors of 1.4 to 4.0, respectively, compared to the parental clone. Binding inhibition assay using flow cytometry and polymerase chain reaction revealed that B2 (6.4 x 10(8) mol/L) had a higher neutralizing activity against pre-S1 or HBV virion binding to liver cell line. This anti-pre-S1 scFv can be considered as a potential therapeutic tool for a passive immunotherapy for HBV infection. | en |
dc.language.iso | en | - |
dc.publisher | Mary Ann Liebert | en |
dc.subject | Amino Acid Sequence | en |
dc.subject | Hepatitis B Antibodies/*immunology | en |
dc.subject | Hepatitis B virus/*immunology/*metabolism | en |
dc.subject | Humans | en |
dc.subject | Immunoglobulin G | en |
dc.subject | Immunoglobulin Variable Region/chemistry/*genetics/*immunology | en |
dc.subject | Molecular Sequence Data | en |
dc.subject | Mutation/*genetics | en |
dc.subject | Neutralization Tests | en |
dc.subject | Protein Binding | en |
dc.subject | Peptide Library | - |
dc.title | Improvement of neutralizing activity of human scFv antibodies against hepatitis B virus binding using CDR3 V(H) mutant library | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 박세광 | - |
dc.contributor.AlternativeAuthor | 정영주 | - |
dc.contributor.AlternativeAuthor | 이영이 | - |
dc.contributor.AlternativeAuthor | 양철민 | - |
dc.contributor.AlternativeAuthor | 김익중 | - |
dc.contributor.AlternativeAuthor | 정준호 | - |
dc.contributor.AlternativeAuthor | 김익상 | - |
dc.contributor.AlternativeAuthor | 이윤재 | - |
dc.contributor.AlternativeAuthor | 박성재 | - |
dc.contributor.AlternativeAuthor | 이정녀 | - |
dc.contributor.AlternativeAuthor | 서수길 | - |
dc.contributor.AlternativeAuthor | 박용홍 | - |
dc.contributor.AlternativeAuthor | 최인학 | - |
dc.identifier.doi | 10.1089/vim.2006.19.115 | - |
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