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Chronic furosemide or hydrochlorothiazide administration increases H+-ATPase B1 subunit abundance in rat kidney
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Na, Ki Young | - |
dc.contributor.author | Kim, Gheun-Ho | - |
dc.contributor.author | Joo, Kwon Wook | - |
dc.contributor.author | Lee, Jay Wook | - |
dc.contributor.author | Jang, Hye Ryoun | - |
dc.contributor.author | Oh, Yun Kyu | - |
dc.contributor.author | Jeon, Un Sil | - |
dc.contributor.author | Chae, Seoung-Wan | - |
dc.contributor.author | Knepper, Mark A | - |
dc.contributor.author | Han, Jin Suk | - |
dc.date.accessioned | 2009-12-30T01:34:00Z | - |
dc.date.available | 2009-12-30T01:34:00Z | - |
dc.date.issued | 2007 | - |
dc.identifier.citation | Am J Physiol Renal Physiol 292:F1701-F1709, 2007 | en |
dc.identifier.issn | 0363-6127 (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17311909 | - |
dc.identifier.uri | https://hdl.handle.net/10371/23408 | - |
dc.description.abstract | Furosemide administration stimulates distal acidification. This has been attributed to the increased lumen-negative voltage in the distal nephron, but the aspect of regulatory mechanisms of H(+)-ATPase has not been clear. The purpose of this study is to investigate whether chronic administration of diuretics alters the expression of H(+)-ATPase and whether electrogenic Na(+) reabsorption is involved in this process. A 7-day infusion of furosemide or hydrochlorothiazide (HCTZ) lowered urine pH significantly. However, this effect of furosemide-induced distal acidification was not changed with amiloride-blocking electrogenic Na(+) reabsorption. On immunoblotting, a polyclonal antibody against the H(+)-ATPase B1 subunit recognized a specific approximately 56-kDa band in membrane fractions from the kidney. The protein abundance of H(+)-ATPase was significantly increased by furosemide and HCTZ infusion in both the cortex and outer medulla. Furosemide plus amiloride administration also increased the H(+)-ATPase protein abundance significantly. However, no definite subcellular redistribution of H(+)-ATPase was observed by furosemide +/- amiloride infusion with immunohistochemistry. Chronic furosemide +/- amiloride administration induced a translocation of pendrin to the apical membrane, while total protein abundance was not increased. The mRNA expression of H(+)-ATPase was not altered by furosemide +/- amiloride infusion. We conclude that chronic administration of diuretics enhances distal acidification by increasing the abundance of H(+)-ATPase irrespective of electrogenic Na(+) reabsorption. This upregulation of H(+)-ATPase in the intercalated cells may be the result of tubular hypertrophy by diuretics. | en |
dc.language.iso | en | en |
dc.publisher | American Physiological Society | en |
dc.subject | Animals | en |
dc.subject | Blotting, Western | en |
dc.subject | Chloride-Bicarbonate Antiporters/metabolism | en |
dc.subject | Diuretics/*pharmacology | en |
dc.subject | Electrophoresis, Polyacrylamide Gel | en |
dc.subject | Furosemide/*pharmacology | en |
dc.subject | Hydrochlorothiazide/*pharmacology | en |
dc.subject | Immunohistochemistry | en |
dc.subject | Kidney/drug effects/*enzymology/pathology | en |
dc.subject | Male | en |
dc.subject | Proton-Translocating ATPases/*biosynthesis/immunology | en |
dc.subject | RNA/biosynthesis | en |
dc.subject | Rats | en |
dc.subject | Rats, Sprague-Dawley | en |
dc.subject | Reverse Transcriptase Polymerase Chain Reaction | en |
dc.subject | Sodium/metabolism | en |
dc.title | Chronic furosemide or hydrochlorothiazide administration increases H+-ATPase B1 subunit abundance in rat kidney | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 나기영 | - |
dc.contributor.AlternativeAuthor | 김근호 | - |
dc.contributor.AlternativeAuthor | 주권욱 | - |
dc.contributor.AlternativeAuthor | 이재욱 | - |
dc.contributor.AlternativeAuthor | 장혜련 | - |
dc.contributor.AlternativeAuthor | 오윤규 | - |
dc.contributor.AlternativeAuthor | 전은실 | - |
dc.contributor.AlternativeAuthor | 채승완 | - |
dc.contributor.AlternativeAuthor | 한진석 | - |
dc.identifier.doi | 10.1152/ajprenal.00270.2006 | - |
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