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Molecular characterization of cDNA encoding porcine IP-10 and induction of porcine endothelial IP-10 in response to human TNF-alpha

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dc.contributor.authorYang, Jaeseok-
dc.contributor.authorChoi, Inho-
dc.contributor.authorKim, Sung Dae-
dc.contributor.authorKim, Eun Sil-
dc.contributor.authorCho, Bumrae-
dc.contributor.authorKim, Jae Young-
dc.contributor.authorAhn, Curie-
dc.date.accessioned2009-12-31T04:50:34Z-
dc.date.available2009-12-31T04:50:34Z-
dc.date.issued2007-03-03-
dc.identifier.citationVet Immunol Immunopathol. 2007 May 15;117(1-2):124-8. Epub 2007 Feb 3.en
dc.identifier.issn0165-2427 (Print)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17328963-
dc.identifier.urihttp://hdl.handle.net/10371/24343-
dc.description.abstractDonor-derived chemokines may play an important role in xenograft rejection, as well as allograft rejection. We have cloned the cDNA encoding porcine IP-10 (interferon-gamma-inducible protein 10), and evaluated its induction in miniature porcine endothelial cells in response to various human cytokines. The cloned sequence is 764 nucleotides long, and the open reading frame consists of 312 nucleotides. The deduced protein sequence includes a predicted mature protein of approximately 83 residues. The comparison of porcine IP-10, and its human, mouse, rat, goat, and sheep counterparts exhibited high similarity among different mammalian species. The sequences of important regulatory elements such as the interferon-stimulated response element (ISRE), and two NF-kappaB binding elements are conserved in the proximal promoter region of porcine IP-10, like other mammalian IP-10s. Human TNF-alpha induced the expression of IP-10 mRNA in porcine endothelial cells, while both human IFN-gamma and human IL-1beta failed. Together, the data of this study suggest that porcine IP-10 may interact with human leukocytes across the species barrier.en
dc.language.isoen-
dc.publisherElsevieren
dc.subjectAmino Acid Sequenceen
dc.subjectAnimalsen
dc.subjectBase Sequenceen
dc.subjectChemokine CXCL10en
dc.subjectChemokines, CXC/biosynthesis/*genetics/immunologyen
dc.subjectCloning, Molecularen
dc.subjectDNA, Complementary/chemistry/*geneticsen
dc.subjectEndothelial Cells/immunologyen
dc.subjectGraft Rejection/immunologyen
dc.subjectHumansen
dc.subjectMolecular Sequence Dataen
dc.subjectRandom Amplified Polymorphic DNA Technique/veterinaryen
dc.subjectSequence Alignmenten
dc.subjectSwineen
dc.subjectSwine, Miniatureen
dc.subjectTumor Necrosis Factor-alpha/immunology/*pharmacologyen
dc.titleMolecular characterization of cDNA encoding porcine IP-10 and induction of porcine endothelial IP-10 in response to human TNF-alphaen
dc.typeArticleen
dc.contributor.AlternativeAuthor양재석-
dc.contributor.AlternativeAuthor최인호-
dc.contributor.AlternativeAuthor김성대-
dc.contributor.AlternativeAuthor김은실-
dc.contributor.AlternativeAuthor조범래-
dc.contributor.AlternativeAuthor김재영-
dc.contributor.AlternativeAuthor안규리-
dc.identifier.doi10.1016/j.vetimm.2007.01.019-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
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