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Screening of LPS-specific peptides from a phage display library using epoxy beads

Cited 35 time in Web of Science Cited 39 time in Scopus
Authors
Kim, Yun-Gon; Lee, Chang-Soo; Chung, Woo-Jae; Kim, Eun-mi; Shin, Dong-Sik; Rhim, Jung-Hyo; Lee, Yoon-Sik; Kim, Byung-Gee; Chung, Junho
Issue Date
2005-02-22
Publisher
Elsevier
Citation
Biochem Biophys Res Commun. 2005 Apr 1;329(1):312-7.
Keywords
Bacteriophages/metabolismBiochemistry/*methodsEnzyme-Linked Immunosorbent AssayEpoxy Resins/*chemistryEscherichia coli/metabolismImmunoassay*Lipopolysaccharides/chemistryMicroscopy, Fluorescence*Peptide LibraryPeptides/*chemistryPolysaccharides/chemistryProtein BindingSalmonella enteritidis/chemistry/metabolismSurface Plasmon Resonance
Abstract
The selection of identical or highly homologous peptides from phage display combinatorial peptide libraries has been unsuccessful in biopanning experiments using microtiter plates. In the present study, by biopanning on LPS-conjugated epoxy beads, we repeatedly enriched clones encoding AWLPWAK and NLQEFLF. These peptides were found to interact with the polysaccharide moiety of LPS, which is highly variable among gram negative bacterial species. In addition, phages encoding these peptides preferentially bound to the LPS of Salmonella family. AWLPWAK-conjugated beads absorbed Salmonella enteritidis from solution and showed a preference for S. enteritidis over Escherichia coli. In summary, this study shows for the first time that a peptide screened from phage displays of combinatorial peptide libraries can be synthesized on beads and be used practically to concentrate bacterial cells from solution.
ISSN
0006-291X (Print)
Language
English
URI
http://hdl.handle.net/10371/24353
DOI
https://doi.org/10.1016/j.bbrc.2005.01.137
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Biochemistry & Molecular Biology (생화학교실)Journal Papers (저널논문_생화학교실)
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