S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Biochemistry & Molecular Biology (생화학교실) Journal Papers (저널논문_생화학교실)
Screening of LPS-specific peptides from a phage display library using epoxy beads
- Kim, Yun-Gon; Lee, Chang-Soo; Chung, Woo-Jae; Kim, Eun-mi; Shin, Dong-Sik; Rhim, Jung-Hyo; Lee, Yoon-Sik; Kim, Byung-Gee; Chung, Junho
- Issue Date
- Biochem Biophys Res Commun. 2005 Apr 1;329(1):312-7.
- Bacteriophages/metabolism; Biochemistry/*methods; Enzyme-Linked Immunosorbent Assay; Epoxy Resins/*chemistry; Escherichia coli/metabolism; Immunoassay; *Lipopolysaccharides/chemistry; Microscopy, Fluorescence; *Peptide Library; Peptides/*chemistry; Polysaccharides/chemistry; Protein Binding; Salmonella enteritidis/chemistry/metabolism; Surface Plasmon Resonance
- The selection of identical or highly homologous peptides from phage display combinatorial peptide libraries has been unsuccessful in biopanning experiments using microtiter plates. In the present study, by biopanning on LPS-conjugated epoxy beads, we repeatedly enriched clones encoding AWLPWAK and NLQEFLF. These peptides were found to interact with the polysaccharide moiety of LPS, which is highly variable among gram negative bacterial species. In addition, phages encoding these peptides preferentially bound to the LPS of Salmonella family. AWLPWAK-conjugated beads absorbed Salmonella enteritidis from solution and showed a preference for S. enteritidis over Escherichia coli. In summary, this study shows for the first time that a peptide screened from phage displays of combinatorial peptide libraries can be synthesized on beads and be used practically to concentrate bacterial cells from solution.
- 0006-291X (Print)
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