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Screening of LPS-specific peptides from a phage display library using epoxy beads
Cited 38 time in
Web of Science
Cited 41 time in Scopus
- Authors
- Issue Date
- 2005-02-22
- Publisher
- Elsevier
- Citation
- Biochem Biophys Res Commun. 2005 Apr 1;329(1):312-7.
- Keywords
- Bacteriophages/metabolism ; Biochemistry/*methods ; Enzyme-Linked Immunosorbent Assay ; Epoxy Resins/*chemistry ; Escherichia coli/metabolism ; Immunoassay ; Microscopy, Fluorescence ; Peptides/*chemistry ; Polysaccharides/chemistry ; Protein Binding ; Salmonella enteritidis/chemistry/metabolism ; Surface Plasmon Resonance ; Lipopolysaccharides/chemistry ; Peptide Library
- Abstract
- The selection of identical or highly homologous peptides from phage display combinatorial peptide libraries has been unsuccessful in biopanning experiments using microtiter plates. In the present study, by biopanning on LPS-conjugated epoxy beads, we repeatedly enriched clones encoding AWLPWAK and NLQEFLF. These peptides were found to interact with the polysaccharide moiety of LPS, which is highly variable among gram negative bacterial species. In addition, phages encoding these peptides preferentially bound to the LPS of Salmonella family. AWLPWAK-conjugated beads absorbed Salmonella enteritidis from solution and showed a preference for S. enteritidis over Escherichia coli. In summary, this study shows for the first time that a peptide screened from phage displays of combinatorial peptide libraries can be synthesized on beads and be used practically to concentrate bacterial cells from solution.
- ISSN
- 0006-291X (Print)
- Language
- English
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