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8-Oxo-7,8-dihydro-2'-deoxyguanosine is not salvaged for DNA synthesis in human leukemic U937 cells

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dc.contributor.authorKim, Ja-Eun-
dc.contributor.authorChung, Myung-Hee-
dc.date.accessioned2009-12-31T05:11:39Z-
dc.date.available2009-12-31T05:11:39Z-
dc.date.issued2006-03-23-
dc.identifier.citationFree Radic Res. 2006 May;40(5):461-6.en
dc.identifier.issn1071-5762 (Print)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16551572-
dc.identifier.urihttps://hdl.handle.net/10371/24403-
dc.description.abstract8-Oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dG), the most common oxidatively modified nucleoside, is released from oxidized DNA and oxidized nucleotide pool. However, little information is available regarding the metabolic pathway of free 8-oxo-dG. In this study, we generated radiolabeled 8-oxo-dG to track its metabolic fate. We report that 8-oxo-dG is neither phosphorylated to 8-oxo-dGMP nor degraded to the free base, 8-oxo-7,8-dihydroguanine (8-oxo-Gua), indicating that 8-oxo-dG is not a substrate for nucleotide synthesis. This result was confirmed by the finding that no radioactivity was detected in the DNA of U937 cells after incubating the cells with radiolabeled 8-oxo-dG. These observations indicate that 8-oxo-dG produced by oxidative stress is not reutilized for DNA synthesis.en
dc.language.isoenen
dc.publisherTaylor & Francisen
dc.subjectCell Line, Tumoren
dc.subjectDNA/*metabolismen
dc.subjectDNA Replication/physiologyen
dc.subjectGuanine/*analogs & derivatives/metabolismen
dc.subjectHumansen
dc.subjectLeukemiaen
dc.subjectOxidative Stress/physiologyen
dc.title8-Oxo-7,8-dihydro-2'-deoxyguanosine is not salvaged for DNA synthesis in human leukemic U937 cellsen
dc.typeArticleen
dc.contributor.AlternativeAuthor김자은-
dc.contributor.AlternativeAuthor정명희-
dc.identifier.doi10.1080/10715760600570539-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Pharmacology (약리학전공)Journal Papers (저널논문_약리학전공)
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