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8-Oxo-7,8-dihydro-2'-deoxyguanosine is not salvaged for DNA synthesis in human leukemic U937 cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Ja-Eun | - |
dc.contributor.author | Chung, Myung-Hee | - |
dc.date.accessioned | 2009-12-31T05:11:39Z | - |
dc.date.available | 2009-12-31T05:11:39Z | - |
dc.date.issued | 2006-03-23 | - |
dc.identifier.citation | Free Radic Res. 2006 May;40(5):461-6. | en |
dc.identifier.issn | 1071-5762 (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16551572 | - |
dc.identifier.uri | https://hdl.handle.net/10371/24403 | - |
dc.description.abstract | 8-Oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dG), the most common oxidatively modified nucleoside, is released from oxidized DNA and oxidized nucleotide pool. However, little information is available regarding the metabolic pathway of free 8-oxo-dG. In this study, we generated radiolabeled 8-oxo-dG to track its metabolic fate. We report that 8-oxo-dG is neither phosphorylated to 8-oxo-dGMP nor degraded to the free base, 8-oxo-7,8-dihydroguanine (8-oxo-Gua), indicating that 8-oxo-dG is not a substrate for nucleotide synthesis. This result was confirmed by the finding that no radioactivity was detected in the DNA of U937 cells after incubating the cells with radiolabeled 8-oxo-dG. These observations indicate that 8-oxo-dG produced by oxidative stress is not reutilized for DNA synthesis. | en |
dc.language.iso | en | en |
dc.publisher | Taylor & Francis | en |
dc.subject | Cell Line, Tumor | en |
dc.subject | DNA/*metabolism | en |
dc.subject | DNA Replication/physiology | en |
dc.subject | Guanine/*analogs & derivatives/metabolism | en |
dc.subject | Humans | en |
dc.subject | Leukemia | en |
dc.subject | Oxidative Stress/physiology | en |
dc.title | 8-Oxo-7,8-dihydro-2'-deoxyguanosine is not salvaged for DNA synthesis in human leukemic U937 cells | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 김자은 | - |
dc.contributor.AlternativeAuthor | 정명희 | - |
dc.identifier.doi | 10.1080/10715760600570539 | - |
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