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8-hydroxydeoxyguanosine suppresses NO production and COX-2 activity via Rac1/STATs signaling in LPS-induced brain microglia
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Hong sook | - |
dc.contributor.author | Ye, Sang-Kyu | - |
dc.contributor.author | Cho, Ik Hyun | - |
dc.contributor.author | Jung, Joo Eun | - |
dc.contributor.author | Kim, Dong-Hyun | - |
dc.contributor.author | Choi, Seongwon | - |
dc.contributor.author | Kim, Yong-Sik | - |
dc.contributor.author | Park, Chung-Gyu | - |
dc.contributor.author | Kim, Tae-Yoon | - |
dc.contributor.author | Lee, Jung Weon | - |
dc.contributor.author | Chung, Myung-Hee | - |
dc.date.accessioned | 2009-12-31T07:55:36Z | - |
dc.date.available | 2009-12-31T07:55:36Z | - |
dc.date.issued | 2006-10-07 | - |
dc.identifier.citation | Free Radic Biol Med. 2006 Nov 1;41(9):1392-403. Epub 2006 Jul 27. | en |
dc.identifier.issn | 0891-5849 (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17023266 | - |
dc.identifier.uri | https://hdl.handle.net/10371/24657 | - |
dc.description.abstract | Free 8-hydroxydeoxyguanosine (oh(8)dG), a nucleoside of 8-hydroxyguanine (oh(8)Gua), present in cytosol is not incorporated into DNA. However, nothing is known about its biological function when it presents in cytosol as a free form. We demonstrate here for the first time that oh(8)dG inhibits lipopolysaccharide (LPS)-induced nitric oxide (NO) production and cyclooxygenase-2 (COX-2) activity, and both gene transcriptions in microglia. Furthermore, oh(8)dG reduced mRNA levels of pro-inflammatory cytokine, such as IL-1beta, IL-6, and TNF-alpha, in activated BV2 cells. We also found that oh(8)dG suppressed reactive oxygen species (ROS) production through reduction of NADPH oxidase activity and blocked Rac1/STATs signal cascade. Finally, oh(8)dG suppressed recruitment of STATs and p300 to the iNOS and COX-2 promoters, and inhibited H3 histone acetylation. Taken together, these results provide new aspects of oh(8)dG as an anti-inflammatory agent. | en |
dc.language.iso | en | en |
dc.publisher | Elsevier | en |
dc.subject | Animals | en |
dc.subject | Blotting, Western | en |
dc.subject | Brain/*drug effects/metabolism | en |
dc.subject | Cells, Cultured | en |
dc.subject | Chromatin Immunoprecipitation | en |
dc.subject | Cyclooxygenase 2/genetics/*metabolism | en |
dc.subject | Deoxyguanosine/*analogs & derivatives/pharmacology | en |
dc.subject | E1A-Associated p300 Protein/metabolism | en |
dc.subject | Lipopolysaccharides/*pharmacology | en |
dc.subject | Luciferases/metabolism | en |
dc.subject | Male | en |
dc.subject | Membrane Proteins/genetics/*metabolism | en |
dc.subject | Mice | en |
dc.subject | Mice, Inbred C57BL | en |
dc.subject | Microglia/cytology/*drug effects/metabolism | en |
dc.subject | Nitric Oxide/*metabolism | en |
dc.subject | Nitrites/metabolism | en |
dc.subject | Promoter Regions, Genetic | en |
dc.subject | RNA, Messenger/genetics/metabolism | en |
dc.subject | Reactive Oxygen Species/metabolism | en |
dc.subject | Reverse Transcriptase Polymerase Chain Reaction | en |
dc.subject | STAT Transcription Factors/*metabolism | en |
dc.subject | Transcription, Genetic | en |
dc.subject | rac1 GTP-Binding Protein/*metabolism | en |
dc.title | 8-hydroxydeoxyguanosine suppresses NO production and COX-2 activity via Rac1/STATs signaling in LPS-induced brain microglia | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 김홍숙 | - |
dc.contributor.AlternativeAuthor | 예상규 | - |
dc.contributor.AlternativeAuthor | 조익현 | - |
dc.contributor.AlternativeAuthor | 정주은 | - |
dc.contributor.AlternativeAuthor | 김동현 | - |
dc.contributor.AlternativeAuthor | 최성원 | - |
dc.contributor.AlternativeAuthor | 김용식 | - |
dc.contributor.AlternativeAuthor | 박정규 | - |
dc.contributor.AlternativeAuthor | 김태윤 | - |
dc.contributor.AlternativeAuthor | 이정원 | - |
dc.contributor.AlternativeAuthor | 정명희 | - |
dc.identifier.doi | 10.1016/j.freeradbiomed.2006.07.018 | - |
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