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Mega-dose Vitamin C modulates T cell functions in Balb/c mice only when administered during T cell activation

Cited 29 time in Web of Science Cited 29 time in Scopus
Authors
Noh, Kahwa; Lim, Hyunja; Moon, Sung-kyu; Kang, Jae Seung; Lee, Wang Jae; Lee, Dongsup; Hwang, Young-il
Issue Date
2005-03-26
Publisher
Elsevier
Citation
Immunol Lett. 2005 Apr 15;98(1):63-72.
Keywords
AnimalsAntioxidants/*pharmacologyAscorbic Acid/*pharmacologyCell ProliferationCytokines/secretionDinitrofluorobenzene/immunologyDrug Hypersensitivity/immunologyHemocyanin/immunologyHypersensitivity, Delayed/chemically induced/immunologyImmunoglobulin E/immunologyImmunoglobulin G/immunologyInflammation/drug therapyLymphocyte Activation/*drug effects/immunologyMiceMice, Inbred BALB CT-Lymphocytes/*drug effects/immunology/secretionTime Factors
Abstract
Previously we reported that a mega-dose of Vitamin C enhanced the initial stage of delayed-type hypersensitivity reaction in Balb/c mice. In this study its effects were further evaluated as follows. Mice were administered Vitamin C intraperitoneally at 0.625 mg/day or at 5mg/day for variable days before, during, or after being sensitized with DNFB. T cells were isolated in each group and examined. When stimulated antigen-specifically or non-specifically in vitro, mice showed elevated thymidine uptake and a shift of cytokine secretion profiles toward Th1, i.e., elevated levels IL-2, TNF-alpha, and IFN-gamma, and lowered level of the Th2 cytokine IL-4, only when Vitamin C was administered during sensitization. T cells from those mice administered Vitamin C before sensitization or after challenge showed the same T cell properties as those from PBS-treated mice. Mice were also given 0.625 mg/day of Vitamin C during primary and/or secondary immunizations with KLH and secondary specific antibody titers in sera were measured. The total specific antibody titer was lowered in Vitamin C-treated animals whenever treatments were administered, and this was entirely attributed to decreased levels of IgG1 and IgE antibodies. Based on these results, we suggest that an exogenously administered mega-dose of Vitamin C shifts immunity in Balb/c mouse toward Th1 and that these affects occur only when Vitamin C is administered during T cell activation.
ISSN
0165-2478 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15790510

https://hdl.handle.net/10371/24760
DOI
https://doi.org/10.1016/j.imlet.2004.10.012
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College of Medicine/School of Medicine (의과대학/대학원)Anatomy (해부학전공)Journal Papers (저널논문_해부학전공)
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