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Angiotensin II inhibits inward rectifier K+ channels in rabbit coronary arterial smooth muscle cells through protein kinase Calpha
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Won Sun | - |
dc.contributor.author | Kim, Nari | - |
dc.contributor.author | Youm, Jae Boum | - |
dc.contributor.author | Warda, Mohamad | - |
dc.contributor.author | Ko, Jae-Hong | - |
dc.contributor.author | Kim, Sung Joon | - |
dc.contributor.author | Earm, Yung E. | - |
dc.contributor.author | Han, Jin | - |
dc.date.accessioned | 2009-12-31T08:35:54Z | - |
dc.date.available | 2009-12-31T08:35:54Z | - |
dc.date.issued | 2006-01-31 | - |
dc.identifier.citation | Biochem Biophys Res Commun. 2006 Mar 17;341(3):728-35. Epub 2006 Jan 19. | en |
dc.identifier.issn | 0006-291X (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16442501 | - |
dc.identifier.uri | https://hdl.handle.net/10371/24778 | - |
dc.description.abstract | We investigated the effects of the vasoconstrictor angiotensin (Ang) II on the whole cell inward rectifier K(+) (Kir) current enzymatically isolated from small-diameter (<100 microm) coronary arterial smooth muscle cells (CASMCs). Ang II inhibited the Kir current in a dose-dependent manner (half inhibition value: 154 nM). Pretreatment with phospholipase C inhibitor and protein kinase C (PKC) inhibitors prevented the Ang II-induced inhibition of the Kir current. The PKC activator reduced the Kir currents. The inhibitory effect of Ang II was reduced by intracellular and extracellular Ca(2+) free condition and by Go6976, which inhibits Ca(2+)-dependent PKC isoforms alpha and beta. However, the inhibitory effect of Ang II was unaffected by a peptide that selectively inhibits the translocation of the epsilon isoform of PKC. Western blot analysis confirmed that PKCalpha, and not PKCbeta, was expressed in small-diameter CASMCs. The Ang II type 1 (AT(1))-receptor antagonist CV-11974 prevented the Ang II-induced inhibition of the Kir current. From these results, we conclude that Ang II inhibits Kir channels through AT(1) receptors by the activation of PKCalpha. | en |
dc.language.iso | en | en |
dc.publisher | Elsevier | en |
dc.subject | Angiotensin II/*pharmacology | en |
dc.subject | Animals | en |
dc.subject | Calcium/chemistry/metabolism | en |
dc.subject | Cations, Divalent/chemistry | en |
dc.subject | Cells, Cultured | en |
dc.subject | Electrophysiology | en |
dc.subject | Female | en |
dc.subject | Isoenzymes/antagonists & inhibitors/classification/metabolism | en |
dc.subject | Male | en |
dc.subject | Muscle, Smooth, Vascular/*drug effects/*metabolism | en |
dc.subject | Myocytes, Smooth Muscle/*drug effects/*metabolism | en |
dc.subject | Patch-Clamp Techniques | en |
dc.subject | Potassium Channels, Inwardly Rectifying/*metabolism | en |
dc.subject | Protein Kinase C-alpha/antagonists & inhibitors/*metabolism | en |
dc.subject | Protein Kinase Inhibitors/pharmacology | en |
dc.subject | Rabbits | en |
dc.subject | Receptor, Angiotensin, Type 1/metabolism | en |
dc.subject | Type C Phospholipases/antagonists & inhibitors/metabolism | en |
dc.title | Angiotensin II inhibits inward rectifier K+ channels in rabbit coronary arterial smooth muscle cells through protein kinase Calpha | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 박원선 | - |
dc.contributor.AlternativeAuthor | 김나리 | - |
dc.contributor.AlternativeAuthor | 염재범 | - |
dc.contributor.AlternativeAuthor | 고재홍 | - |
dc.contributor.AlternativeAuthor | 김성준 | - |
dc.contributor.AlternativeAuthor | 엄융의 | - |
dc.contributor.AlternativeAuthor | 한진 | - |
dc.identifier.doi | 10.1016/j.bbrc.2006.01.026 | - |
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