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Role of Src-specific phosphorylation site on focal adhesion kinase for senescence-associated apoptosis resistance
Cited 28 time in
Web of Science
Cited 27 time in Scopus
- Authors
- Issue Date
- 2006-03-09
- Publisher
- Springer Verlag
- Citation
- Apoptosis 2006; 11: 303-313
- Keywords
- Apoptosis/*physiology ; Caspase 3/metabolism ; Cell Cycle ; Cells, Cultured ; Collagen Type XI/metabolism ; Enzyme Activation ; Enzyme Inhibitors/pharmacology ; Fibroblasts/cytology/drug effects/physiology ; Focal Adhesion Protein-Tyrosine Kinases/genetics/*metabolism ; Humans ; Hydrogen Peroxide/pharmacology ; Oxidants/pharmacology ; Phosphorylation ; RNA, Small Interfering/metabolism ; Staurosporine/pharmacology ; Tyrosine/metabolism ; src-Family Kinases/antagonists & inhibitors/*metabolism ; Cell Aging
- Abstract
- A decreased apoptotic response toward noxious stress is an issuing characteristic of the aging phenotype. Hydrogen peroxide or staurosporine induced apoptosis readily in young cells but not in senescent cells. We showed that focal adhesion kinase (FAK) expression and its phosphorylation at Tyr397, autophosphorylation site for focal adhesion formation, and Tyr577, Src-dependent phosphorylation site, were both increased in senescent cells. Moreover, FAK was inactivated proteolytically by apoptotic stimuli in young cells, but not in senescent cells. In addition, senescent cells whose FAK expression was downregulated by siRNA showed the increased level of apoptosis by staurosporine treatment via caspase-3 activation but not by hydrogen peroxide treatment. Interestingly dephosphorylation at Tyr577 of FAK by PP2 treatment, Src-family kinase inhibitor, induced the apoptosis by staurosporine in senescent cells but dephosphorylation at Tyr397 by downregulation of caveolin-1 was not affected. These data suggest that FAK might differently regulate apoptosis and focal adhesion formation through site-specific tyrosine phosphorylation in senescent cells.
- ISSN
- 1360-8185 (Print)
- Language
- English
- URI
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16523241
https://hdl.handle.net/10371/24808
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