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Short hairpin RNA causes the methylation of transforming growth factor-beta receptor II promoter and silencing of the target gene in rat hepatic stellate cells

Cited 27 time in Web of Science Cited 34 time in Scopus
Authors
Kim, Jin-Wook; Zhang, Yan-Hong; Zern, Mark A; Rossi, John J; Wu, Jian
Issue Date
2007
Publisher
Elsevier
Citation
Biochem Biophys Res Commun 2007;359:292-297
Keywords
AnimalsAzacitidine/analogs & derivatives/pharmacologyBase SequenceCell LineCpG Islands*DNA MethylationGene SilencingLiver/*cytologyMolecular Sequence DataNucleotides/chemistry*Promoter Regions, GeneticProtein-Serine-Threonine KinasesRNA, Small Interfering/*geneticsRatsReceptors, Transforming Growth Factor beta/*geneticsSequence Analysis, DNASequence Homology, Nucleic AcidTranscription, Genetic
Abstract
Small interfering RNA (siRNA) induces transcriptional gene silencing (TGS) in plant and animal cells. RNA dependent DNA methylation (RdDM) accounts for TGS in plants, but it is unclear whether siRNA induces RdDM in mammalian cells. To determine whether stable expression of short hairpin siRNA (shRNA) induces DNA methylation in mammalian cells, we transduced rat hepatic stellate SBC10 cells with lentiviral vectors which encode an U6 promoter-driven shRNA expression cassette homologous to the transforming growth factor-beta receptor (TGFbetaRII) promoter region. Sequencing analysis of bisulfite-modified genomic DNA showed the methylation of cytosine residues both in CpG dinucleotides and non-CpG sites around the target region of the TGFbetaRII promoter in SBC10 cells transduced with the promoter-targeting lentiviral vector. In these cells, real-time RT-PCR showed a decrease in TGFbetaRII mRNA levels which were reversed by treatment with 5-aza-2-deoxycytidine. Our results demonstrate that recombinant lentivirus-mediated shRNA delivery resulted in the methylation of the homologous promoter area in mammalian cells, and this approach may be used as a tool for transcriptional gene silencing by epigenetic modification of mammalian cell promoters.
ISSN
0006-291X (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17533113

https://hdl.handle.net/10371/24837
DOI
https://doi.org/10.1016/j.bbrc.2007.05.080
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College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
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